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Participation at least during 80% of the course is essential for practice fulfillment.
In the case of non-fulfillment of the credit conditions, the student can obtain a credit on the basis of a seminar work. Specifics of the seminar work are discussed with teacher individually. If the student could not attend the class, for example due to a disease, a medical report can be accepted instead of the student’s attendance.
Poslední úprava: Balušíková Kamila, RNDr., Ph.D. (29.09.2017)
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The study is performed in the form of practices Poslední úprava: Balušíková Kamila, RNDr., Ph.D. (29.09.2017)
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A) Analysis of nucleic acids: 1) DNA and RNA isolation 2) Measurement of DNA and RNA concentration 3) PCR 4) Methods of mutations detection (RFLP, PCR with allele specific primers) 5) Electrophoresis of DNA and RNA 6) Real-time PCR* 7) Detection of microRNA* 8) DNA sequencing*
B) Analysis of proteins: 1) Proteins isolation 2) Measurement of proteins concentration 3) Electrophoresis of proteins (SDS-PAGE) 4) Western blot analysis 5) Fluorescent microscopy 6) ELISA* 7) Flow cytometry*
*only theoretical lessons
Poslední úprava: Balušíková Kamila, RNDr., Ph.D. (29.09.2017)
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