Stem Cells and Regenerative Medicine - FV045

• Anglický název: Stem Cells and Regenerative Medicine
• Zajišťuje: Ústav histologie a embryologie (15-140)
• Fakulta: Lékařská fakulta v Hradci Králové
• Platnost: od 2022
• Semestr: letní
• Body: 0
• E-Kredity: 2
• Způsob provedení zkoušky: letní s.:
• Rozsah, examinace: letní s.:25/7, Z [HT]
• Počet míst: 35
• Minimální obsazenost: 5
• 4EU+: ne
• Virtuální mobilita / počet míst pro virtuální mobilitu: ne
• Stav předmětu: vyučován
• Jazyk výuky: angličtina
• Způsob výuky: prezenční
• Způsob výuky: prezenční
• Poznámka: předmět je možno zapsat mimo plán; povolen pro zápis po webu
• Garant: prof. MUDr. Jaroslav Mokrý, Ph.D.; MUDr. Tomáš Soukup, Ph.D.
• Patří mezi: General medicine - ELECTIVE subjects
• Prerekvizity : {Malá skupina pro FV045}
• Neslučitelnost : FG50019
• Záměnnost : FG50019
• Je neslučitelnost pro: FG50019
• Je záměnnost pro: FG50019

Anotace

Poslední úprava: Markéta Ulrichová (17.01.2024)

In lectures, students gain knowledge in a progressively developing field of stem cell biology and regenerative medicine incl. closely related areas of tissue engineering and cell therapy. In the course of practical classes, students become familiar with detection methods and basic procedures in the tissue culture laboratory.

Vstupní požadavky

Poslední úprava: Markéta Ulrichová (17.01.2024)

Students who have passed histology, embryology and biology are eligible to register.

Cíl předmětu

Poslední úprava: Markéta Ulrichová (17.01.2024)

Outcomes:

1. the student acquires theoretical knowledge ofa progressively developing field of stem cell biology and regenerative medicine incl. closely related areas of tissue engineering, IVF and cell therapy.
2. in the course of practical classes, students become familiar with SCs isolation procedures, detection methods and basic techniques in the tissue culture laboratory.

Požadavky ke zkoušce

Poslední úprava: Markéta Ulrichová (17.01.2024)

Conditions for obtaining credits:

Presence in lectures and laboratory classes. Absences may be substituted only by writing an assay on the topic you missed. Elaboration of protocol for cell viability.

Sylabus

Poslední úprava: Markéta Ulrichová (17.01.2024)

Lecturers:

• prof. MUDr. Stanislav Filip, Ph.D.
• prof. MUDr. Jaroslav Mokrý, Ph.D.
• prof. MUDr. Pavel Žák, Ph.D.
• MUDr. Tomáš Soukup, Ph.D.
• doc. MUDr. Jakub Suchánek, Ph.D.

Lectures:

1. Introduction. Regenerative medicine, regeneration, reparation. Cell therapy. Stem and progenitor cells - basic terms, characterization, biological behaviour, differentiation potential. Stem cell plasticity. Telomeres, telomerase and ageing of stem cells. Ethics and moral aspects of stem cell research.
2. Pluripotent stem cells, ES cells, iPS cells. Epithelial stem cells. Tumour stem cells. Muscle-derived stem cells; muscle regeneration.
3. Neural stem cells and their niche. Regeneration of the nervous tissue. Spinal cord injury.
4. Endothelial progenitor cells. Haematopoietic stem cells. Haematological applications, bone marrow transplantation.
5. Mesenchymal stem cells. Dental pulp stem cells. Separation techniques. Cellular interactions. Growth factors.
6. Scaffolds (2D, 3D) - natural, artificial. Encapsulation. Nanotechnology. Cellular migration. Cell differentiation. Cell transplantation. Tissue engineering of bones and cartilages. Orthopaedic applications.

Practical courses:

1. Tissue engineering. Laboratory facilities and basic procedures in tissue culture laboratory. Safety aspects, risk assessment. Preparation of buffers and media. Phase contrast microscopy.
2. Cultivation techniques. Isolation of cells from human and animal tissues. Enzymatic tissue dissociation. Cell quantification. Cryoconservation. Mathematics in the laboratory - number of population doublings, doubling time, kinetics.
3. Cell isolation. Primary cultures. Subculturing. Cultivation surfaces. Basic biological characteristics. Cell separation techniques. Gradient centrifugation (Ficoll, Percoll). Immunomagnetic separation.
4. Cultivation protocols. Stem cell differentiation in vitro. Extracellular matrix analysis.
5. Visit of tissue culture laboratory. Cell viability. Preparation 

Literatura

Poslední úprava: Markéta Ulrichová (17.01.2024)

Recommended literature:

1. Alberts et al. Molecular Biology of the Cell, W. W. Norton & Company; Sixth edition (November 18, 2014), ISBN 9780815344322
2. Atala et al. Handbook of Stem Cells, Academic Press; 2 edition (October 8, 2012), ISBN 9780123859426
3. Wingard et al. Hematopoietic Stem Cell Transplantation: A Handbook for Clinicians, 2nd edition, American Association of Blood Banks (AABB); 2nd edition (January 28, 2015), ISBN 1563958767
4. Clark et al. The Molecular and Cellular Biology of Wound Repair, Springer; 1988 edition (October 24, 2012), ISBN 146135725X
5. Zhou et al. Mammalian Cell Cultures for Biologics Manufacturing (Advances in Biochemical Engineering/Biotechnology), Springer; 2014 edition (January 16, 2014), ISBN 364254049X
6. Bronzino et al. The Biomedical Engineering Handbook: Four Volume Set, CRC Press; 4 edition (April 10, 2015), ISBN 1439825335
7. Ratner et al. Biomaterials Science: An Introduction to Materials in Medicine, Academic Press; 3 edition (November 8, 2012), ISBN 9780123746269
8. Frank H. Stephenson Calculations for Molecular Biology and Biotechnology, Academic Press, Elsevier Science 2003, ISBN 0-12-665751-3