Practical Course of DNA diagnostics Annotation: The aim of this practical course is to introduce recent basic
techniques used in DNA diagnostics. After theoretical opening all students will have the possibility to try practically
basic methods in lab. Course is designed for highly motivated students of 3rd to 6th year of general medicine
studies, who are thinking about PhD studies; and is open also for PhD. students. Course is open for 6 students;
takes 5-6 hours of two afternoons, and takes place in Laboratoř pro studium mitochondrialnich poruch, at the Dep.
of Pediatrics (Klinika dětského a dorostového lékařství), Ke Karlovu 2, Praha 2, building E4. Notice: The exact date
is arranged individually due to the signed students’ possibilities. Attendants please contact Iveta Tothova, PhD. by
e-mail: iveta.tothova@lf1.cuni.cz (contact phone No. 224967748).
Sylabus - angličtina
Poslední úprava: Marcela Holanová (29.04.2021)
Syllabus:
Practical course of DNA diagnostics.
Scope of course: theoretical opening; DNA isolation (classical methods and commercial kits) from different tissues (blood, hair, buccal cells, cultivated fibroblasts), concentration measurement (NanoDrop), methods of DNA amplification (PCR- practically; cloning - theoretically), mutation detection methods - implications and applications (practically: ELFO, RFLP; theoretically: sequencing, HA, DGGE). Molecular results interpretation.
Your questions, please, address to: iveta.tothova@lf1.cuni.cz or call 22496 7748.
Metody výuky - angličtina
Poslední úprava: Ing. Markéta Tesařová, Ph.D. (23.10.2023)
Date: 14. - 15.12.2023 (12:30 - 18:00)
Place: Laborator pro studium mitochondriálních poruch, which is part of the Department of Pediatrics anf Inherited Metabolic Disorders (Ke Karlovu 2), in building E4. The entrance is from the right side of the building. Ring the bell "Mitolab Prijem materialu" and come to the third floor. Please, takeyour lab coat with you.
