In lectures, students gain knowledge in a progressively developing field of stem cell biology and regenerative medicine incl. closely related areas of tissue engineering and cell therapy. In the course of practical classes, students become familiar with detection methods and basic procedures in the tissue culture laboratory.
Vstupní požadavky - angličtina
Poslední úprava: Markéta Ulrichová (17.01.2024)
Students who have passed histology, embryology and biology are eligible to register.
Cíl předmětu - angličtina
Poslední úprava: Markéta Ulrichová (17.01.2024)
Outcomes:
1. the student acquires theoretical knowledge ofa progressively developing field of stem cell biology and regenerative medicine incl. closely related areas of tissue engineering, IVF and cell therapy. 2. in the course of practical classes, students become familiar with SCs isolation procedures, detection methods and basic techniques in the tissue culture laboratory.
Požadavky ke zkoušce - angličtina
Poslední úprava: Markéta Ulrichová (17.01.2024)
Conditions for obtaining credits:
Presence in lectures and laboratory classes. Absences may be substituted only by writing an assay on the topic you missed. Elaboration of protocol for cell viability.
Sylabus - angličtina
Poslední úprava: Markéta Ulrichová (17.01.2024)
Lecturers:
prof. MUDr. Stanislav Filip, Ph.D.
prof. MUDr. Jaroslav Mokrý, Ph.D.
prof. MUDr. Pavel Žák, Ph.D.
MUDr. Tomáš Soukup, Ph.D.
doc. MUDr. Jakub Suchánek, Ph.D.
Lectures:
Introduction. Regenerative medicine, regeneration, reparation. Cell therapy. Stem and progenitor cells - basic terms, characterization, biological behaviour, differentiation potential. Stem cell plasticity. Telomeres, telomerase and ageing of stem cells. Ethics and moral aspects of stem cell research.
Tissue engineering. Laboratory facilities and basic procedures in tissue culture laboratory. Safety aspects, risk assessment. Preparation of buffers and media. Phase contrast microscopy.
Cultivation techniques. Isolation of cells from human and animal tissues. Enzymatic tissue dissociation. Cell quantification. Cryoconservation. Mathematics in the laboratory - number of population doublings, doubling time, kinetics.
Cultivation protocols. Stem cell differentiation in vitro. Extracellular matrix analysis.
Visit of tissue culture laboratory. Cell viability. Preparation
Literatura - angličtina
Poslední úprava: Markéta Ulrichová (17.01.2024)
Recommended literature:
Alberts et al. Molecular Biology of the Cell, W. W. Norton & Company; Sixth edition (November 18, 2014), ISBN 9780815344322
Atala et al. Handbook of Stem Cells, Academic Press; 2 edition (October 8, 2012), ISBN 9780123859426
Wingard et al. Hematopoietic Stem Cell Transplantation: A Handbook for Clinicians, 2nd edition, American Association of Blood Banks (AABB); 2nd edition (January 28, 2015), ISBN 1563958767
Clark et al. The Molecular and Cellular Biology of Wound Repair, Springer; 1988 edition (October 24, 2012), ISBN 146135725X
Zhou et al. Mammalian Cell Cultures for Biologics Manufacturing (Advances in Biochemical Engineering/Biotechnology), Springer; 2014 edition (January 16, 2014), ISBN 364254049X
Bronzino et al. The Biomedical Engineering Handbook: Four Volume Set, CRC Press; 4 edition (April 10, 2015), ISBN 1439825335
Ratner et al. Biomaterials Science: An Introduction to Materials in Medicine, Academic Press; 3 edition (November 8, 2012), ISBN 9780123746269
Frank H. Stephenson Calculations for Molecular Biology and Biotechnology, Academic Press, Elsevier Science 2003, ISBN 0-12-665751-3