Obsahem biochemie a patobiochemie stomatologického směru budou základní poznatky o přeměně látek v organismu ve vztahu k nejdůležitějším a nejčastějším nemocem se kterými se stomatolog při své praxi může setkat (diabetes, poruchy hemokoagulace). Podrobně budou probrány zejména trávicí procesy, obrana organismu proti infekci (zánět) a metabolismus pojiva a tvrdých tkání (zuby, kosti ). Zvláštní pozornost bude věnována metabolismu vápníku a jeho poruchám a dále biochemii a patobiochemii ústní dutiny (sliny, zubní plak).
Poslední úprava: Vladěna Topičová (22.09.2007)
Biochemistry and pathobiochemistry for dentists will be concentrated on the fundamentals of metabolism related to the most important and most frequent diseases which may be encountered by the dentist (e.g. hemocoagulation disturbances, diabetes etc.). In detail will be discussed especially the digestion processes, defence against infection (inflammation) and metabolism of connective and hard tissues (bone and tooth). The metabolism of calcium and its disturbances will be especially stressed. The biochemistry and pathobiochemistry of oral cavity (salive, dental plaque) will be stressed too.
Sylabus - angličtina
Poslední úprava: Jana Kolářová (31.01.2020)
Lectures
General features of metabolism. Bioenergetics.
Digestion, absorption, storage and transport of saccharides.
The citric acid cycle.
Regulation of metabolism of saccharides.
Biomembranes. Composed lipids.
Lipoproteins: Classification, properties and metabolism.
Steroids: Synthesis and metabolism. Steroid hormones. Bile acids.
Conversion of amino acids to biologically active derivatives.
The connective tissue, bone and cartilage. Proteoglycans, collagen and elastin - metabolism.
Saliva.
Seminars
Biologic oxidation, mitochondrial respiratory chain and oxidative phosphorylation
Stereochemistry of saccharides
Metabolism of saccharides.
Overview of basic methods used in biochemical lab: photometry, chromatography, electrophoresis, blotting.
Lipids: Biosynthesis and degradation of fatty acids
Relationships between metabolism of lipids and saccharides. Starvation
Metabolism of amino acids: Transamination, deamination, synthesis of urea.
Porphyrins and bile pigments
Metabolism of calcium, phosphate, and other elements and vitamins necessary for mineralisation of bone and teeth.
Tooth: Chemical composition and structure
Practical lessons
Saccharides I: Colored reactions of saccharides. Analysis of unknown saccharide.
Saccharides II: Acidic hydrolysis of starch. Substrate specificity of amylase and sucrase. Polarimetry of glucose and fructose.
Introduction to spectrophotometry: Absorption spectra of colored solutions. Calibration curve, factor calculation.
Saccharides III: Estimation of glycemia. Glucose in urine.
Saliva: Demonstration of selected components
Lipids: Estimation of total cholesterol and triacylglycerols. Demonstration of double bonds in fatty acids. Test for malondialdehyde. Ketone bodies in urine.
Liver tests: Direct and indirect bilirubin. Estimation of aminotransferases.
Bone and teeth: Estimation of calcium and phosphate in serum and urine. Isoenzymes of alkaline phosphatase. Properties of dental hydroxyapatite.
Literatura
Poslední úprava: MUDr. Lenka Fialová, CSc. (26.09.2019)
P.C. Champe, R.A. Harvey, and D.R. Ferrier: Lippincott s Illustrated Reviews: Biochemistry. Lippincott Williams & Wilkins (latest edition)
B. Alberts et al.: Essential Cell Biology. Garland Science Publishing, New York (latest edition)
R.K. Murray et al.: Harper?s Illustrated Biochemistry. McGraw-Hill, Inc., USA (latest edition)
Podmínky zakončení předmětu - angličtina
Poslední úprava: MUDr. Lenka Fialová, CSc. (16.05.2019)
Credit requirements:
Sufficient attendance to the seminars and practical lessons. At most three seminars or practical lessons (altogether) are allowed to be missed in one term from serious and certifiable reasons.
Passing the Revision test on the core metabolic pathways (announced separately). Two more chances to retake the test can be provided.
Completed, handed in, marked and approved reports from the practical lessons.
Passing the Practical Credit Examination (announced separately). Two more chances to retake the practical examination can be provided.
Good knowledge of the subject matters covered by the course.
Rozpis výuky -
Rozpis blokový
Týden bloku
Den
Od - Do
Typ výuky
Téma
Učitel
Soubory
Poznámka
Hodnocení
1
Úterý
13:30 - 16:00
nic
Anatomical Dissections
2
Úterý
13:30 - 16:00
nic
Anatomical Dissections
3
Úterý
13:30 - 15:00
seminář
Biologic oxidation, mitochondrial respiratory chain and oxidative phosphorylation
MUDr. Jan Pláteník, Ph.D.
4
Úterý
13:30 - 15:00
seminář
Stereochemistry of saccharides
MUDr. Jan Pláteník, Ph.D.
Pátek
14:15 - 16:30
praktická cvičení
Saccharides I: Colored reactions of saccharides.
Analysis of unknown saccharide.
MUDr. Jan Pláteník, Ph.D.
5
Úterý
13:30 - 15:00
seminář
Metabolism of saccharides.
MUDr. Jan Pláteník, Ph.D.
Pátek
14:15 - 16:30
praktická cvičení
Saccharides II: Acidic hydrolysis of starch. Substrate specificity of amylase and sucrase.
Polarimetry of glucose and fructose.
MUDr. Jan Pláteník, Ph.D.
6
Úterý
13:30 - 15:00
seminář
Overview of basic methods used in biochemical lab: photometry, chromatography, electrophoresis, blotting.
MUDr. Jan Pláteník, Ph.D.
Pátek
14:15 - 16:30
nic
Friday 29.3. Easter Holiday
7
Úterý
13:30 - 15:00
seminář
Lipids: Biosynthesis and degradation of fatty acids
doc. MUDr. Evžen Křepela, CSc.
Pátek
14:15 - 16:30
praktická cvičení
Introduction to spectrophotometry: Absorption spectra of colored solutions. Calibration curve, factor calculation.
MUDr. Jan Pláteník, Ph.D.
8
Úterý
13:30 - 15:00
seminář
Relationships between metabolism of lipids and saccharides. Starvation
MUDr. Jan Pláteník, Ph.D.
Pátek
14:15 - 16:30
praktická cvičení
Saccharides III: Estimation of glycemia. Glucose in urine.
MUDr. Jan Pláteník, Ph.D.
9
Úterý
13:30 - 15:00
seminář
Metabolism of amino acids: Transamination, deamination, synthesis of urea.
MUDr. Jan Pláteník, Ph.D.
Pátek
14:15 - 16:30
praktická cvičení
Lipids: Estimation of total cholesterol and triacylglycerols. Demonstration of double bonds in fatty acids. Test for malondialdehyde.
MUDr. Jan Pláteník, Ph.D.
10
Úterý
13:30 - 15:00
seminář
Revision test: Core metabolic pathways
MUDr. Jan Pláteník, Ph.D.
11
Úterý
13:30 - 15:00
seminář
Porphyrins and bile pigments
doc. MUDr. Evžen Křepela, CSc.
Pátek
14:15 - 16:30
praktická cvičení
Liver tests: Direct and indirect bilirubin. Estimation of aminotransferases.
MUDr. Jan Pláteník, Ph.D.
12
Úterý
13:30 - 15:00
seminář
Metabolism of calcium, phosphate, and other elements and vitamins necessary for mineralisation of bone and teeth.
doc. MUDr. Ivan Matouš-Malbohan, CSc.
Pátek
14:15 - 16:30
praktická cvičení
Bone and teeth: Estimation of calcium and phosphate in serum and urine. Isoenzymes of alkaline phosphatase. Properties of dental hydroxyapatite.
MUDr. Jan Pláteník, Ph.D.
13
Úterý
13:30 - 15:00
seminář
Tuesday 14.5. Rector's Day
MUDr. Jan Pláteník, Ph.D.
Pátek
14:15 - 16:30
praktická cvičení
Saliva: Demonstration of selected components
MUDr. Jan Pláteník, Ph.D.
14
Úterý
13:30 - 15:00
seminář
Tooth: Chemical composition and structure
doc. MUDr. Ivan Matouš-Malbohan, CSc.
Pátek
14:15 - 16:30
praktická cvičení
Practical credit test, credit
MUDr. Jan Pláteník, Ph.D.
15
Pátek
14:15 - 16:30
praktická cvičení
Credit
Rozpis semestrální
Výukový týden
Datum
Od - Do
Typ výuky
Téma
Učitel
Soubory
Poznámka
Hodnocení
1
20.02.2024
12:30 - 13:15
nic
Anatomical Dissections
2
27.02.2024
12:30 - 13:15
nic
Anatomical Dissections
3
05.03.2024
12:30 - 13:15
přednáška
General features of metabolism. Bioenergetics.
MUDr. Jan Pláteník, Ph.D.
4
12.03.2024
12:30 - 13:15
přednáška
The citric acid cycle.
MUDr. Jan Pláteník, Ph.D.
5
19.03.2024
12:30 - 13:15
přednáška
Digestion, absorption, storage and transport of saccharides.
MUDr. Jan Pláteník, Ph.D.
6
26.03.2024
12:30 - 13:15
přednáška
Regulation of metabolism of saccharides.
MUDr. Jan Pláteník, Ph.D.
7
02.04.2024
12:30 - 13:15
přednáška
Biomembranes. Composed lipids.
RNDr. Marek Vecka, Ph.D.
8
09.04.2024
12:30 - 13:15
přednáška
Lipoproteins: Classification, properties and metabolism.
MUDr. Martin Vejražka, Ph.D.
9
16.04.2024
12:30 - 13:15
přednáška
Steroids: Synthesis and metabolism. Steroid hormones. Bile acids.
RNDr. Marek Vecka, Ph.D.
10
23.04.2024
12:30 - 13:15
nic
11
30.04.2024
12:30 - 13:15
přednáška
Conversion of amino acids to biologically active derivatives.
MUDr. Jan Krtil
12
07.05.2024
12:30 - 13:15
přednáška
The connective tissue, bone and cartilage. Proteoglycans, collagen and elastin – metabolism.
doc. MUDr. Ivan Matouš-Malbohan, CSc.
13
14.05.2024
12:30 - 13:15
přednáška
Saliva
doc. MUDr. Ivan Matouš-Malbohan, CSc.
Schedule block
Week of the block
Day
From - To
Education type
Theme
Teacher
Files
Note
Hodnocení
1
Tuesday
13:30 - 16:00
nothing
Anatomical Dissections
2
Tuesday
13:30 - 16:00
nothing
Anatomical Dissections
3
Tuesday
13:30 - 15:00
seminar
Biologic oxidation, mitochondrial respiratory chain and oxidative phosphorylation
MUDr. Jan Pláteník, Ph.D.
4
Tuesday
13:30 - 15:00
seminar
Stereochemistry of saccharides
MUDr. Jan Pláteník, Ph.D.
Friday
14:15 - 16:30
practical classes
Saccharides I: Colored reactions of saccharides.
Analysis of unknown saccharide.
MUDr. Jan Pláteník, Ph.D.
5
Tuesday
13:30 - 15:00
seminar
Metabolism of saccharides.
MUDr. Jan Pláteník, Ph.D.
Friday
14:15 - 16:30
practical classes
Saccharides II: Acidic hydrolysis of starch. Substrate specificity of amylase and sucrase.
Polarimetry of glucose and fructose.
MUDr. Jan Pláteník, Ph.D.
6
Tuesday
13:30 - 15:00
seminar
Overview of basic methods used in biochemical lab: photometry, chromatography, electrophoresis, blotting.
MUDr. Jan Pláteník, Ph.D.
Friday
14:15 - 16:30
nothing
Friday 29.3. Easter Holiday
7
Tuesday
13:30 - 15:00
seminar
Lipids: Biosynthesis and degradation of fatty acids
doc. MUDr. Evžen Křepela, CSc.
Friday
14:15 - 16:30
practical classes
Introduction to spectrophotometry: Absorption spectra of colored solutions. Calibration curve, factor calculation.
MUDr. Jan Pláteník, Ph.D.
8
Tuesday
13:30 - 15:00
seminar
Relationships between metabolism of lipids and saccharides. Starvation
MUDr. Jan Pláteník, Ph.D.
Friday
14:15 - 16:30
practical classes
Saccharides III: Estimation of glycemia. Glucose in urine.
MUDr. Jan Pláteník, Ph.D.
9
Tuesday
13:30 - 15:00
seminar
Metabolism of amino acids: Transamination, deamination, synthesis of urea.
MUDr. Jan Pláteník, Ph.D.
Friday
14:15 - 16:30
practical classes
Lipids: Estimation of total cholesterol and triacylglycerols. Demonstration of double bonds in fatty acids. Test for malondialdehyde.
MUDr. Jan Pláteník, Ph.D.
10
Tuesday
13:30 - 15:00
seminar
Revision test: Core metabolic pathways
MUDr. Jan Pláteník, Ph.D.
11
Tuesday
13:30 - 15:00
seminar
Porphyrins and bile pigments
doc. MUDr. Evžen Křepela, CSc.
Friday
14:15 - 16:30
practical classes
Liver tests: Direct and indirect bilirubin. Estimation of aminotransferases.
MUDr. Jan Pláteník, Ph.D.
12
Tuesday
13:30 - 15:00
seminar
Metabolism of calcium, phosphate, and other elements and vitamins necessary for mineralisation of bone and teeth.
doc. MUDr. Ivan Matouš-Malbohan, CSc.
Friday
14:15 - 16:30
practical classes
Bone and teeth: Estimation of calcium and phosphate in serum and urine. Isoenzymes of alkaline phosphatase. Properties of dental hydroxyapatite.
MUDr. Jan Pláteník, Ph.D.
13
Tuesday
13:30 - 15:00
seminar
Tuesday 14.5. Rector's Day
MUDr. Jan Pláteník, Ph.D.
Friday
14:15 - 16:30
practical classes
Saliva: Demonstration of selected components
MUDr. Jan Pláteník, Ph.D.
14
Tuesday
13:30 - 15:00
seminar
Tooth: Chemical composition and structure
doc. MUDr. Ivan Matouš-Malbohan, CSc.
Friday
14:15 - 16:30
practical classes
Practical credit test, credit
MUDr. Jan Pláteník, Ph.D.
15
Friday
14:15 - 16:30
practical classes
Credit
Schedule semestral
Educational week
Date
From - To
Education type
Theme
Teacher
Files
Note
Hodnocení
1
20.02.2024
12:30 - 13:15
nothing
Anatomical Dissections
2
27.02.2024
12:30 - 13:15
nothing
Anatomical Dissections
3
05.03.2024
12:30 - 13:15
lecture
General features of metabolism. Bioenergetics.
MUDr. Jan Pláteník, Ph.D.
4
12.03.2024
12:30 - 13:15
lecture
The citric acid cycle.
MUDr. Jan Pláteník, Ph.D.
5
19.03.2024
12:30 - 13:15
lecture
Digestion, absorption, storage and transport of saccharides.
MUDr. Jan Pláteník, Ph.D.
6
26.03.2024
12:30 - 13:15
lecture
Regulation of metabolism of saccharides.
MUDr. Jan Pláteník, Ph.D.
7
02.04.2024
12:30 - 13:15
lecture
Biomembranes. Composed lipids.
RNDr. Marek Vecka, Ph.D.
8
09.04.2024
12:30 - 13:15
lecture
Lipoproteins: Classification, properties and metabolism.
MUDr. Martin Vejražka, Ph.D.
9
16.04.2024
12:30 - 13:15
lecture
Steroids: Synthesis and metabolism. Steroid hormones. Bile acids.
RNDr. Marek Vecka, Ph.D.
10
23.04.2024
12:30 - 13:15
nothing
11
30.04.2024
12:30 - 13:15
lecture
Conversion of amino acids to biologically active derivatives.
MUDr. Jan Krtil
12
07.05.2024
12:30 - 13:15
lecture
The connective tissue, bone and cartilage. Proteoglycans, collagen and elastin – metabolism.