Advanced Laboratory Course in Analytical Chemistry - MC230C21E

• Anglický název: Advanced Laboratory Course in Analytical Chemistry
• Zajišťuje: Katedra analytické chemie (31-230)
• Fakulta: Přírodovědecká fakulta
• Platnost: od 2024
• Semestr: oba
• E-Kredity: 6
• Rozsah, examinace: 0/2, Z [TS]
• Rozsah za akademický rok: 2 [týdny]
• Počet míst: neomezen
• Minimální obsazenost: neomezen
• 4EU+: ne
• Virtuální mobilita / počet míst pro virtuální mobilitu: ne
• Stav předmětu: vyučován
• Jazyk výuky: angličtina
• Poznámka: při zápisu přednost, je-li ve stud. plánu; předmět lze zapsat v ZS i LS
• Garant: RNDr. Jana Sobotníková, Ph.D.
• Vyučující: RNDr. Václav Červený, Ph.D.; RNDr. Hana Dejmková, Ph.D.; Anton Korban, Ph.D.; RNDr. Eliška Nováková, Ph.D.; RNDr. Jana Sobotníková, Ph.D.; prof. RNDr. Vlastimil Vyskočil, Ph.D.
• Neslučitelnost : MC230C10, MC230C13, MC230C15
• Je neslučitelnost pro: MC230C15, MC230C10, MC230C13

Anotace

This practical laboratory course introduces students to the conventional instrumental techniques of chemical analysis. The analytical methods taught are divided into 3 basic groups: electrochemical, separation and spectrometric methods. Emphasis is placed on understanding the principles demonstrated for each instrumental method. Students are guided through the handling of raw experimental data, its statistical evaluation and the presentation of calculated results. Strict compliance with GLP and safety regulations is required throughout the course. Upon completing the course, students should be able to work independently with common analytical instruments. An integral part of the classification is the continuous control of the knowledge required to perform each task, the classification of protocols and the evaluation of laboratory work.

This laboratory course is organized in two-week sessions with students working in small groups. This laboratory course is organised in the summer and winter terms. IN THE WINTER TERM, THE COURSE TAKES PLACE AT THE END OF NOVEMBER OR IN DECEMBER (2-WEEK BLOCK).

Poslední úprava: Sobotníková Jana, RNDr., Ph.D. (12.06.2024)

Literatura

1. Task Instructions and Safety Regulations can be downloaded from SIS.

2. D. A. Skoog, F. J. Holler, S. R. Crouch, Principles of Instrumental Analysis, 7th ed., 2017
3. D. C. Harris, Quantitative Chemical Analysis, 10th ed., 2019
4. D. A. Skoog, D. M. West, F. J. Holler, S. R. Crouch, Fundamentals of Analytical Chemistry, 10th ed., 2022

Poslední úprava: Sobotníková Jana, RNDr., Ph.D. (12.06.2024)

Požadavky ke zkoušce

Credits will be awarded on completion of all prescribed tasks in the course and submission of all properly prepared protocols within 14 days of the end of the course.

Protocols will be graded from 1 (excellent) to 4 (unsatisfactory) according to their quality (results, statistical analysis and presentation).

Credits will be awarded to students whose sum of all grades from oral examinations (including 4 and grades from corrective examinations) does not exceed 25 and whose sum of all grades from protocols (also including 4 and grades from revised protocols) does not exceed 25.

If the sum of the grades from the oral examinations or protocols exceeds 25, an additional oral examination will be required for credit. If the sum of the grades from the oral examinations or protocols exceeds 30, no course credit can be awarded. Re-enrolment in the course is required to receive credit.

Poslední úprava: Sobotníková Jana, RNDr., Ph.D. (22.01.2025)

Sylabus

The following exercises are done:

1. Classical DC polarography
2. Karl Fischer titrations (KF)
3. Spectrophotometry - Determination of dissociation constant of an acid-base indicator
4. Determination of  Bi³⁺ and Cd²⁺ ions with AAS and K⁺ ion using Flame photometry
5. Gas chromatography (GC) - Qualitative and quantitative analysis
6. Determination of azodyes in a mixture using RP-HPLC method with spectrophotometric detection
7. Conductometry and Conductometric titration
8. Capillary zone electrophoresis (CZE)

Poslední úprava: Sobotníková Jana, RNDr., Ph.D. (12.06.2024)

Výsledky učení

On completion of the course, the student will be able to: for these instrumental analytical methods (polarography, conductometry and conductometric titration, Karl Fischer titration, atomic absorption and emission spectrometry, spectrophotometry, capillary electrophoresis, gas chromatography with flame ionization detection, liquid chromatography with UV/VIS detection):

- explain the principle of the above analytical methods

- independently prepare solutions and use instruments (carry out measurements) based on task instructions

- interpret measured graphs and curves (polarographic curves, conductometric and potentiometric titration curves, absorption/emission spectra, chromatograms, calibration curves)

- describe, evaluate and draw conclusions from measured data

- record the procedure, results and conclusions in a report

Poslední úprava: Sobotníková Jana, RNDr., Ph.D. (12.06.2024)

Vstupní požadavky

Students should have knowledge about principles of electrochemical, separation and spectrometric analytical methods. In addition, evaluation of raw experimental data, their statistical evaluation, and presentation of calculated results are necessary input requirements for attending this course. 

Poslední úprava: Sobotníková Jana, RNDr., Ph.D. (12.06.2024)

Požadavky k zápisu

Cannot be enrolled at the same time as MC230C13, MC230C10 and MC230C21E.

Poslední úprava: Sobotníková Jana, RNDr., Ph.D. (12.06.2024)