Pokročilý laboratorní kurz zaměřený na morfologii, ultrastrukturu a determinaci parazitických prvoků. Posluchači se budou moci seznámit s celou řadou parazitických protist formou demonstrace živých kultur, pitev hostitelů a především barvených trvalých preparátů.
Poslední úprava: Hampl Vladimír, doc. Mgr., Ph.D. (17.03.2019)
Advanced laboratory course focused on morphology, ultrastructure and determination of parasitic protists. Parasitic protists will be demonstrated in the form of living cultures, dissections of hosts and mainly in the form on fixed and stained preparations.
Poslední úprava: Hampl Vladimír, doc. Mgr., Ph.D. (11.02.2025)
Literatura -
Aktuální sylaby, schemata a metodické návody průběžně poskytované během kurzu.
Poslední úprava: Kulíková Helena, RNDr. (18.03.2019)
Literature:
Current syllabi, schematics and methodological instructions provided during the course.
Poslední úprava: Hampl Vladimír, doc. Mgr., Ph.D. (17.03.2019)
Požadavky ke zkoušce -
Požadavkem zápočtu je účast na praktiku, úspěšné složení závěrečné poznávací zkoušky (mikroskopické preparáty, interpretace fotografií z elektronového mikroskopu)a znalost principu metod prováděných na praktiku.
Poslední úprava: Hampl Vladimír, doc. Mgr., Ph.D. (11.02.2025)
The requirement for credits is the participation in the course, successful completion of the determination of the test set of microscopic slides, interpretation of photographs from the electron microscope and knowledge of the principles of methods performed on the practice.
Poslední úprava: Hampl Vladimír, doc. Mgr., Ph.D. (11.02.2025)
Sylabus -
20.2. Introduction, Kinetoplastea I. Basic ultrastructure of euglenozoa, characteristics of the main groups. Single-host kinetoplastids. Morphological forms in the developmental cycles of trypanosomatids.
6.3. Kinetoplastea II. Pathogenic trypanosomes and leishmania, vector and vertebrate stages, microscopic determination of the main representatives.
13.3. Diplomonadida and Retortamonadida. Ultrastructure, demonstration of the main representatives.
20.3. Praeaxostyla and Parabasalia. Demonstration of pathogenic trichomonads of humans and animals. Morphological differentiation of pathogenic and non-pathogenic human trichomonads.
27.3. Pathogenic amphizoid amoebae. Naegleria, Acanthamoeba, Balamuthia. Ultrastructure, demonstration of the main representatives.
10.4. Human intestinal amoebae. Entamoeba histolytica. Ultrastructure, demonstration of the main representatives. Microscopic differentiation of human intestinal amoebae.
17.4 Gregarines and Cryptosporidium. Basic ultrastructure of the apicomplexus and peculiarities of gregarines and cryptosporidia. Ultrastructure, demonstration of the main representatives.
24.4. Coccidia:Eimeria, Toxoplasma, Sarcocystis, Frenkelia. Determination of developmental stages of coccidia in histological preparations.
15.5. Haemosporida and Piroplasmida. Ultrastructure and developmental cycles. Haemosporinae of birds (Haemoproteus. Plasmodium, Leukocytozoon). Identification of theileria and babesia on blood smears and tissue impressions. Malaria agents and their microscopic diagnosis. Determination of parasitaemia.
This lecture (Microspora) is voluntary. After the lecture, Dr. Hyliš will be available for consultation.
5. 5 Microspora. Ultrastructure and developmental cycles of basic genera. Diagnosis and preparation of specimens of selected species from infected insects and crustaceans. Demonstration of permanent preparations of microsporidia from fish, mammals and man. Opportunistic human microsporidioses and their diagnosis. Myxozoa, opalines and funnels. Demonstration of preparations.
Poslední úprava: Rada Petr, RNDr., Ph.D. (12.02.2026)
20.2. Introduction, Kinetoplastea I. Basic ultrastructure of euglenozoa, characteristics of the main groups. Single-host kinetoplastids. Morphological forms in the developmental cycles of trypanosomatids.
6.3. Kinetoplastea II. Pathogenic trypanosomes and leishmania, vector and vertebrate stages, microscopic determination of the main representatives.
13.3. Diplomonadida and Retortamonadida. Ultrastructure, demonstration of the main representatives.
20.3. Praeaxostyla and Parabasalia. Demonstration of pathogenic trichomonads of humans and animals. Morphological differentiation of pathogenic and non-pathogenic human trichomonads.
27.3. Pathogenic amphizoid amoebae. Naegleria, Acanthamoeba, Balamuthia. Ultrastructure, demonstration of the main representatives.
10.4. Human intestinal amoebae. Entamoeba histolytica. Ultrastructure, demonstration of the main representatives. Microscopic differentiation of human intestinal amoebae.
17.4 Gregarines and Cryptosporidium. Basic ultrastructure of the apicomplexus and peculiarities of gregarines and cryptosporidia. Ultrastructure, demonstration of the main representatives.
24.4. Coccidia:Eimeria, Toxoplasma, Sarcocystis, Frenkelia. Determination of developmental stages of coccidia in histological preparations.
15.5. Haemosporida and Piroplasmida. Ultrastructure and developmental cycles. Haemosporinae of birds (Haemoproteus. Plasmodium, Leukocytozoon). Identification of theileria and babesia on blood smears and tissue impressions. Malaria agents and their microscopic diagnosis. Determination of parasitaemia.
This lecture (Microspora) is voluntary. After the lecture, Dr. Hyliš will be available for consultation.
5. 5 Microspora. Ultrastructure and developmental cycles of basic genera. Diagnosis and preparation of specimens of selected species from infected insects and crustaceans. Demonstration of permanent preparations of microsporidia from fish, mammals and man. Opportunistic human microsporidioses and their diagnosis. Myxozoa, opalines and funnels. Demonstration of preparations.
Poslední úprava: Rada Petr, RNDr., Ph.D. (12.02.2026)
Výsledky učení - angličtina
Main Course Outcomes
1. Structural Understanding Students will be able to describe and compare the ultrastructure of major protist groups, including Kinetoplastea, Amoebozoa, Apicomplexa, Microspora, and related taxa.
2. Life Cycle Analysis Students will be able to explain and interpret key life cycles and developmental stages of medically and veterinary relevant protists, including trypanosomes, Leishmania, coccidia, haemosporidians, piroplasms, and microsporidians.
3. Microscopic Identification Skills Students will be able to perform accurate microscopic identification of diagnostic stages of pathogenic protists in blood smears, tissue impressions, histological preparations, and permanent slides.
4. Differential Diagnostic Competence Students will be able to distinguish pathogenic from non‑pathogenic and closely related protist species (e.g., trichomonads, intestinal amoebae) using morphological and developmental criteria.
Poslední úprava: Rada Petr, RNDr., Ph.D. (02.02.2026)
