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Credit: Attendance in lectures and practicals
Poslední úprava: Čechová Jana, Mgr. (13.09.2018)
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No exam Poslední úprava: Heřman Petr, RNDr. (02.11.2017)
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Introduction to tissue engineering
Tissue structure - overview Selected techniques and methods applied in tissue and protein engineering - summary (lasers, fluorescence, flow cytometry, FACS, electromigration and chromatographic methods, PCR, spectroscopic and spectrometric methods) Regenerative medicine and tissue engineering Cells in tissue engineering - principle sources, cells isolated from tissues, embryonic and adult stem cells Tissue engineering Cartilage tissue engineering Bone tissue engineering Blood-vessel tissue engineering Skin covers Nanofibers, their production and safety Nanofibers and production methods Safety of nanofibers and other nanosystems Medical textiles as scaffolds for cell seeding Reasons and methods of nanofiber functionalization
Bio-nanotechnology and systems of controlled drug release Application of bio-nanotechnology in tissue engineering and regenerative medicine Controlled drug delivery, liposomes, their preparation and application „Smart nanofiber/liposome systems“ for controlled delivery of bioactive molecules Bio-nanotechnology for artificial tissue production Exogenous and endogenous cell sources Bio-nanotechnology and mobilization of endogenous cell sources Biomechanical properties on native and artificial tissues and their determination Quality testing of bio-implants (in vitro and in vivo tests) Experimental part A. Nanofibers and controlled drug delivery (UCEEB CTU) Task 1: Preparation of nanofibers by electrospinning and forcespinning methods Task 2: Visualization of nanofiber's morphology (SEM analysis) Task 3: Evaluation of physico-chemical properties of nanofibers (FTIR) Task 4: Demonstration of 3D printing method B. In vitro characterization of cells (IEM ASCR) Task 1: Cell passaging Task 2: Cell staining, confocal microscopy - DiOC6 and propidium iodide staining Task 3: Cells metabolic activity testing - MTS test Task 4: Visiting the GMP laboratory for human cell and tissue therapy at IBC ASCR Poslední úprava: Čechová Jana, Mgr. (13.09.2018)
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