PředmětyPředměty(verze: 964)
Předmět, akademický rok 2024/2025
   Přihlásit přes CAS
OS - Tissue engineering and nanobiotechnology in regenerative medicine - DVA1096
Anglický název: OS - Tissue engineering and nanobiotechnology in regenerative medicine
Zajišťuje: Ústav biofyziky (13-714)
Fakulta: 2. lékařská fakulta
Platnost: od 2023
Semestr: zimní
Body: 3
E-Kredity: 3
Způsob provedení zkoušky: zimní s.:
Rozsah, examinace: zimní s.:0/20, Z [HS]
Počet míst: neurčen / 18 (18)
Minimální obsazenost: 2
4EU+: ne
Virtuální mobilita / počet míst pro virtuální mobilitu: ne
Kompetence:  
Stav předmětu: vyučován
Jazyk výuky: angličtina
Způsob výuky: prezenční
Úroveň:  
Poznámka: předmět je možno zapsat mimo plán
povolen pro zápis po webu
Garant: Mgr. Eva Filová, Ph.D.
Vyučující: prof. RNDr. Evžen Amler, CSc.
Mgr. Radek Divín
Mgr. Eva Filová, Ph.D.
Aleksei Pashchenko
Viktorie Ročková, M.Sc.
Podmínky zakončení předmětu -
Credit: Attendance in lectures and practicals

Poslední úprava: Čechová Jana, Mgr. (13.09.2018)
Požadavky ke zkoušce - angličtina

No exam

Poslední úprava: Heřman Petr, RNDr. (02.11.2017)
Sylabus -
Introduction to tissue engineering
Tissue structure - overview

Selected techniques and methods applied in tissue and protein engineering - summary (lasers, fluorescence, flow cytometry, FACS, electromigration and chromatographic methods, PCR, spectroscopic and spectrometric methods)

Regenerative medicine and tissue engineering

Cells in tissue engineering - principle sources, cells isolated from tissues, embryonic and adult stem cells

Tissue engineering
Cartilage tissue engineering

Bone tissue engineering

Blood-vessel tissue engineering

Skin covers

Nanofibers, their production and safety
Nanofibers and production methods

Safety of nanofibers and other nanosystems

Medical textiles as scaffolds for cell seeding

Reasons and methods of nanofiber functionalization

Bio-nanotechnology and systems of controlled drug release

Application of bio-nanotechnology in tissue engineering and regenerative medicine

Controlled drug delivery, liposomes, their preparation and application

„Smart nanofiber/liposome systems“ for controlled delivery of bioactive molecules

Bio-nanotechnology for artificial tissue production
Exogenous and endogenous cell sources

Bio-nanotechnology and mobilization of endogenous cell sources

Biomechanical properties on native and artificial tissues and their determination

Quality testing of bio-implants (in vitro and in vivo tests)

Experimental part
A. Nanofibers and controlled drug delivery (UCEEB CTU)
Task 1: Preparation of nanofibers by electrospinning and forcespinning methods

Task 2: Visualization of nanofiber's morphology (SEM analysis)

Task 3: Evaluation of physico-chemical properties of nanofibers (FTIR)

Task 4: Demonstration of 3D printing method

B. In vitro characterization of cells (IEM ASCR)
Task 1: Cell passaging

Task 2: Cell staining, confocal microscopy - DiOC6 and propidium iodide staining

Task 3: Cells metabolic activity testing - MTS test

Task 4: Visiting the GMP laboratory for human cell and tissue therapy at IBC ASCR

Poslední úprava: Čechová Jana, Mgr. (13.09.2018)
 
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