Stem Cells and Regenerative Medicine - FG50019

• Anglický název: Stem Cells and Regenerative Medicine
• Zajišťuje: Ústav histologie a embryologie (15-140)
• Fakulta: Lékařská fakulta v Hradci Králové
• Platnost: od 2022
• Semestr: letní
• Body: 0
• E-Kredity: 2
• Způsob provedení zkoušky: letní s.:
• Rozsah, examinace: letní s.:25/7, Z [HT]
• Počet míst: 35
• Minimální obsazenost: 5
• 4EU+: ne
• Virtuální mobilita / počet míst pro virtuální mobilitu: ne
• Stav předmětu: nevyučován
• Jazyk výuky: angličtina
• Způsob výuky: prezenční
• Způsob výuky: prezenční
• Poznámka: předmět je možno zapsat mimo plán; povolen pro zápis po webu
• Garant: prof. MUDr. Jaroslav Mokrý, Ph.D.; MUDr. Tomáš Soukup, Ph.D.
• Neslučitelnost : FV045
• Prerekvizity : FG10004
• Záměnnost : FV045
• Je neslučitelnost pro: FV045
• Je záměnnost pro: FV045

Anotace

čeština

Poslední úprava: Markéta Ulrichová (04.01.2022)

Předmět navazuje na znalosti cytologie. Studenty seznamuje s diskutovanými tématy současnosti, jakými bezpochyby jsou: kmenové buňky, buněčné kultury v biomedicíně, buněčná terapie, regenerativní medicína, tkáňové inženýrství apod. V praktických cvičeních jsou studenti obeznámeni se základy práce v laboratoři tkáňových kultur a s detekčními metodami.

angličtina

Poslední úprava: Markéta Ulrichová (04.01.2022)

Students who have passed histology, embryology and biology are eligible to register. In lectures, students gain knowledge in a progressively developing field of stem cell biology and regenerative medicine incl. closely related areas of tissue engineering and cell therapy. In the course of practical classes, students become familiar with detection methods and basic procedures in the tissue culture laboratory.

Požadavky ke zkoušce

Poslední úprava: Markéta Ulrichová (04.01.2022)

Credit

Presence in lectures and laboratory classes. Absences must be substituted by a written essay on the missed topic. Elaboration of protocol for cell viability.

Sylabus

Poslední úprava: Markéta Ulrichová (10.01.2022)

Lectures

Lecturers
  Prof. Jaroslav Mokry, M.D., Ph.D.
  Prof. Stanislav Filip, M.D., Ph.D.
  Assoc. Prof. Pavel Zak, M.D., Ph.D.
  Tomas Soukup, M.D., Ph.D.

 

1    Introduction. Regenerative medicine, regeneration, reparation. Cell therapy. Stem and progenitor cells - basic terms, characterization, biological behaviour, differentiation potential. Stem cell plasticity. Telomeres, telomerase and ageing of stem cells. Ethics and moral aspects of stem cell research. (Mokry, 3)
2    Pluripotent stem cells, ES cells, iPS cells. Epithelial stem cells. Tumour stem cells. Muscle-derived stem cells; muscle regeneration. (Mokry, 3)

3    Neural stem cells and their niche. Regeneration of the nervous tissue. Spinal cord injury. (Mokry, 3) 
4    Endothelial progenitor cells. Haematopoietic stem cells. Haematological applications, bone marrow transplantation. (Mokry, 1; Filip, 1; Žák, 1) 

5    Mesenchymal stem cells. Dental pulp stem cells. Separation techniques. Cellular interactions. Growth factors. (Soukup, 3) 
6    Scaffolds (2D, 3D) - natural, artificial. Encapsulation. Nanotechnology. Cellular migration. Cell differentiation. Cell transplantation. Tissue engineering of bones and cartilages Orthopaedic applications. (Soukup, 3) 

 
Practical courses and seminars

Lecturers
  Tomas Soukup, M.D., Ph.D.
  Assoc. Prof. Jakub Suchanek, M.D., Ph.D.

 
Practical courses
1 Tissue engineering. Laboratory facilities and basic procedures in tissue culture laboratory. Safety aspects, risk assessment. Preparation of buffers and media. Phase contrast microscopy.
2 Cultivation techniques. Isolation of cells from human and animal tissues. Enzymatic tissue dissociation. Cell quantification. Cryoconservation. Mathematics in the laboratory - number of population doublings, doubling time, kinetics.
3 Cell isolation. Primary cultures. Subculturing. Cultivation surfaces. Basic biological characteristics. Cell separation techniques. Gradient centrifugation (Ficoll, Percoll). Immunomagnetic separation.
4 Cultivation protocols. Stem cell differentiation in vitro. Extracellular matrix analysis.
5 Visit of tissue culture laboratory. Cell viability. Preparation of cells for flow cytometric analysis.

 

Literatura

Poslední úprava: Markéta Ulrichová (04.01.2022)

Recommended  literature

1. Alberts et al Molecular Biology of the Cell, Garland, ISBN 0815340729
 
2. Sell, S. Stem Cell Handbook, Humana Press, 2004,4th Editoion,2002, E-ISBN 1-59259-411-5
 
3. Turksen, K. Adult Stem Cells, Humana Press, 2004, E-ISBN 1-59259-732-7
 
4. Clark et al. The Molecular and Cellular Biology of Wound Repair, Plenum Press, 1996, ISBN 030645159X
 
5. Doyle et al. Mammalian Cell Culture (Essential Techniques), John Wiley and Sons, 1997, ISBN 0-471-97057-3
 
6. Bronzino et al. The Biomedical Engineering Handbook, CRC Press, 1999
 
7. Ratner et al. Biomaterial Science, Academic Press, 1997, ISBN 012824610
 
8. Patrick et al. Frontiers in Tissue Engineering, Pergamon, 1998, ISBN 0080426891
 
9. Lanza et al. Principles of Tissue Engineering, Academic Press, 2000, ISBN 0124366309

10. Frank H. Stephenson Calculations for Molecular Biology and Biotechnology, Academic Press, Elsevier Science 2003, ISBN 0-12-665751-3