Experimental Methods in Chemistry - MC260P147

• Title: Experimental Methods in Chemistry
• Guaranteed by: Department of Physical and Macromolecular Chemistry (31-260)
• Faculty: Faculty of Science
• Actual: from 2023 to 2023
• Semester: summer
• E-Credits: 5
• Examination process: summer s.:
• Hours per week, examination: summer s.:2/2, C+Ex [HT]
• Capacity: unlimited
• Min. number of students: unlimited
• 4EU+: no
• Virtual mobility / capacity: no
• State of the course: not taught
• Language: English
• Note: enabled for web enrollment
• Guarantor: prof. Ing. Jiří Čejka, DrSc.

Annotation

Last update: RNDr. Kateřina Ušelová, Ph.D. (31.01.2022)

The course Experimental Methods in Chemistry aims to provide a general introduction to the principles and
methods, which are basically applied in chemical laboratory.
The course set the knowledge base for understanding of the principles of different experimental techniques starting
with crystallization, distillation, or pH measurement, up to diffraction methods, microscopy, various spectroscopic
techniques, nuclear magnetic resonance, etc.
The course also covers separation methods including chromatography, electrochemical methods or calorimetry.

Literature

Last update: RNDr. Kateřina Ušelová, Ph.D. (26.05.2022)

J. Lynch – Physico-Chemical Analysis of Industrial Catalysts, TECHNIP 2003 J.W. Niemantsverdriet – Spectroscopy in Catalysis, VCH 1993 D.W. Bennett - Understanding Single-Crystal X-Ray Crystallography, Wiley, 2010 C. Giacovazzo - Fundamentals of Crystallography, Oxford University Press, 2011 C.J. Chen - Introduction to Scanning Tunneling Microscopy, Oxford University Press, 2008. B. Voigtlander - Scanning Probe Microscopy: Atomic Force Microscopy and Scanning Tunneling Microscopy, 2015 T.D. Claridge: High-resolution NMR Techniques in Organic Chemistry, Elsevier, 2009 D.C. Apperley, R.K. Harris, P. Hodgkinson: Solid-state NMR, Basic Principles and Practice, MomentumPress, 2012 D.B. Williams, C.B. Carter - Transmission Electron Microscopy - A Textbook for Materials Science, 2009 J.M. Thompson – Infrared Spectroscopy, Taylor and Francis 2018 W.C. Sanders - Atomic Force Microscopy, Fundamental Concepts and Laboratory Investigations, Taylor and Francis 2020

Requirements to the exam

Last update: RNDr. Kateřina Ušelová, Ph.D. (31.01.2022)

The final mark is based on the oral examination (67%) and the results of tests taken during the course (33%). The oral examination takes place during the examination period and students must first obtain the credit for practical exercises. Credit for exercises is based on the solution of take-home problems (34%) and two tests (midterm and final, each 33%).

Syllabus

Last update: RNDr. Kateřina Ušelová, Ph.D. (31.01.2022)

• Introduction. Goals of studies. What is the potential of the most important methods. Which techniques and approaches are available. Which information we want to get and how we can get it ?

• Basic principles of Experimental techniques - crystallization, distillation, pH measurement, rectification

• Definitions of experimental techniques - Spectroscopy, Chromatography, Diffraction, Microscopy, NMR, Electrochemical methods, Calorimetry, Surface methods.

• Diffraction - principles of the method, which problems could be solved, X-ray diffraction, electron diffraction, neutron diffraction - different setup, synchrotron. Powder vs. monocrystal techniques.

• Spectroscopy - IR, UV-vis, Raman. Principles of the techniques, information to be extracted. Experimental arrangement, in situ - operando

• (Magic angle spinning) Nuclear magnetic resonance - basic principles and applications - liquid vs. solid phase

• Separation and Chromatography - principles, applications. Gas chromatography, Liquid chromatography, Size exclusion chromatography

• Mass spectrometry - Electrochemical methods

• Microscopy - Principles of Microscopy. Scanning Probe Microscopy, Scanning and Transmission Electron microscopy, Atomic Force Microscopy. General introduction, basic mechanisms of imaging

• Microscopy - SPM and chemistry, chemical identification, molecular charge states, on-surface reactions and molecular manipulation

• Photoemission methods - X-ray photoelectron spectroscopy, Auger spectroscopy