Last update: RNDr. Kateřina Ušelová, Ph.D. (07.02.2022)
The course Chemistry Laboratory aims to provide a general introduction to
the practical aspects of methods in chemical laboratory.
The students should get a knowledge on basis of organic and inorganic synthesis including crystallization,
distillation, or pH measurement. Introduction to diffraction methods, microscopy, spectroscopy, nuclear magnetic
resonance, separation methods including chromatography, and electrochemical methods will be provided.
Literature
Last update: RNDr. Kateřina Ušelová, Ph.D. (26.05.2022)
J. Lynch – Physico-Chemical Analysis of Industrial Catalysts, TECHNIP 2003
J.W. Niemantsverdriet – Spectroscopy in Catalysis, VCH 1993
C. Giacovazzo - Fundamentals of Crystallography, Oxford University Press 2011
T.D. Claridge: High-resolution NMR Techniques in Organic Chemistry, Elsevier, 2009
D.B. Williams, C.B. Carter - Transmission Electron Microscopy - A Textbook for Materials Science, 2009
J.M. Thompson – Infrared Spectroscopy, Taylor and Francis 2018
Requirements to the exam
Last update: RNDr. Kateřina Ušelová, Ph.D. (02.02.2022)
100 % participation in laboratory. Successful passing of two tests. Acceptance of laboratory reports.
Syllabus
Last update: RNDr. Kateřina Ušelová, Ph.D. (02.02.2022)
Introduction. Goals of studies. Good laboratory practice. Safety in laboratory. Basic principles of inorganic synthesis - filtration, centrifugation, pH measurement
Basic principles of inorganic synthesis - crystallization, preparation of a crystal material, autoclaves, X-ray diffraction
Basic principles of organic synthesis - crystallization, distillation, determination of melting points
Basic principles of organic synthesis - preparation of compounds, which will be characterized by different methods
Synthesis of golden nanoparticles and their characterization by TEM
Characterization of golden nanoparticles in solution (hydrodynamic radius, electrophoretic mobility) by ZetaSizer and UV-Vis spectroscopy
Basic principles of organic synthesis - column separation of natural compounds
Calorimetry
Liquid NMR - measurement and determination of structure of compounds prepared ad 4) and 6)
FTIR Spectroscopy - preparation of samples - KBr, self-supporting wafers methods, determination of acidity of sample prepared ad 2)
Catalytic experiment - samples prepared ad 2) quick experiment in a liquid phase followed by gas chromatography and GC/MS
