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Proteins - organic chemistry and biochemistry - MC250P28

Title:	Proteiny - organickochemické a biochemické aspekty
Czech title:	Proteiny - organickochemické a biochemické aspekty
Guaranteed by:	Department of Biochemistry (31-250)
Faculty:	Faculty of Science
Actual:	from 2022
Semester:	summer
E-Credits:	3
Examination process:	summer s.:
Hours per week, examination:	summer s.:2/0, Ex [HT]
Capacity:	unlimited
Min. number of students:	unlimited
4EU+:	no
Virtual mobility / capacity:	no
State of the course:	taught
Language:	Czech
Note:	enabled for web enrollment

Guarantor:	prof. RNDr. Helena Ryšlavá, CSc.
Teacher(s):	RNDr. Kateřina Bělonožníková, Ph.D. prof. RNDr. Helena Ryšlavá, CSc.

Opinion survey results Examination dates SS schedule

Annotation -

Last update: RNDr. Kateřina Bělonožníková, Ph.D. (18.02.2024)

Proteins, method of their isolation and characterization, primary structure; affinity technique in the study of binding properties of proteins.
Lectures - in Czech -

Literature - Czech

Last update: RNDr. Kateřina Bělonožníková, Ph.D. (18.02.2024)

Bioanalytics: Analytical Methods and Concepts in Biochemistry and Molecular Biology, Edited by Friedrich Lottspeich and Joachim W. Engels, Wiley-VCH, 2018

Principles and Techniques of Practical Biochemistry, Keith Wilson and John Walker, Cambridge University Press, Fifth edition, 2000.

Electrophoresis in Practice, Reiner Westermeier, Wiley‐VCH Verlag GmbH & Co. KGaA, 2004.

Biochemistry: R.H. Garrett, C.M. Grisham, Harcourt Brace College Publishers, Fort WORTH, 1995

Proteins: Structure and Molecular Properties: T.E. Creighton, W.H. Freeman 1992

Requirements to the exam - Czech

Last update: RNDr. Kateřina Bělonožníková, Ph.D. (18.02.2024)

Ústní zkouška v rozsahu sylabu.

Syllabus -

Last update: RNDr. Kateřina Bělonožníková, Ph.D. (18.02.2024)

The course summarizes basic protein isolation techniques, different types of separation techniques and methods used for protein characterization.

1. Properties of amino acids
2. Primary structure of proteins
3. Isolation of proteins
4. Gel chromatography
5. Ionex, hydrophobic chromatography
6. Affinity, multimodal chromatography
7. Electrokinetic methods
8. Immunochemical detection of proteins
9. Proteomic analysis