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Enzymology - MC250P01

Title:	Enzymologie
Czech title:	Enzymologie
Guaranteed by:	Department of Biochemistry (31-250)
Faculty:	Faculty of Science
Actual:	from 2023
Semester:	summer
E-Credits:	2
Examination process:	summer s.:
Hours per week, examination:	summer s.:2/0, Ex [HT]
Capacity:	unlimited
Min. number of students:	unlimited
4EU+:	no
Virtual mobility / capacity:	no
State of the course:	not taught
Language:	Czech
Is provided by:	MC250P01A
Note:	enabled for web enrollment

Guarantor:	prof. RNDr. Petr Hodek, CSc.
Teacher(s):	RNDr. Helena Dračínská, Ph.D.
Incompatibility :	MC250P01A
Is incompatible with:	MC250P01A

Opinion survey results Examination dates Schedule

Annotation -

Last update: SIMONAT (22.04.2002)

Enzyme classification and nomenclature. Methods of enzyme isolation and determination. Kinetics of enzyme reaction (Michaelis-Menten kinetics, inhibition, two substrate reactions). Influence of pH and temperature. Regulatory enzymes. Reaction mechanism of selected enzymes.

Literature -

Last update: RNDr. Helena Dračínská, Ph.D. (29.10.2019)

Macholán L., Barthová J., Kučera I.: Enzymologie (vydavatelství MU Brno, 1994)

Kodíček M.: Studijní materiály z enzymologie (vydavatelství VŠCHT Praha, 2003)

Vodrážka Z., Rauch P., Káš J.: Enzymologie (vydavatelství VŠCHT Praha, 1998)

Kučera I.: Řešené úlohy z enzymologie (vydavatelství UJEP Brno, 1987)

Price N. C., Stevens L.: Fundamentals of Enzymology (Oxford University Press, 1999)

Bisswanger H.: Enzyme Kinetics (Wiley-VCH, 2008)

Cornish-Bowden A.: Fundamentals of Enzyme Kinetics (Wiley-Blackwell, 2012)

Copeland R. A.: Enzymes - A Practical Introduction to Structure, Mechanism, and Data Analysis (Wiley-VCH, 2000)

Voet D., Voet J. G.: Biochemistry (Wiley, 2011) / Biochemie (Victoria Publishing, 1996) - kapitola Mechanismy působení enzymů (Mechanisms of Enzyme Action)

Syllabus -

Last update: RNDr. Helena Dračínská, Ph.D. (02.04.2018)

Enzyme classification and nomenclature. Methods of enzyme isolation. Methods of enzyme analysis. General kinetic principles, the steady state enzyme kinetics. Kinetics of enzyme reactions with one or more intermediates. Inhibition of enzyme reaction - pure and mixed inhibition types, inhibition by substrate. Two substrate reactions. Influence of pH and temperature on kinetics of enzyme reaction. Regulatory enzymes - allosteric enzymes, covalently modulated enzymes, isoenzymes. Mechanism of enzyme reactions (theory and hypothesis). Reaction mechanism of selected enzymes. Application of enzymes.