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Mass spectrometry in biopolymer analysis - MC230P70

Title:	Hmotnostní spektrometrie v analýze biopolymerů
Czech title:	Hmotnostní spektrometrie v analýze biopolymerů
Guaranteed by:	Department of Analytical Chemistry (31-230)
Faculty:	Faculty of Science
Actual:	from 2021
Semester:	summer
E-Credits:	3
Examination process:	summer s.:combined
Hours per week, examination:	summer s.:2/0, Ex [HT]
Capacity:	unlimited
Min. number of students:	unlimited
4EU+:	no
Virtual mobility / capacity:	no
State of the course:	taught
Language:	Czech
Explanation:	1.9.2021 dodatečně nastaven jako vyučovaný
Note:	enabled for web enrollment

Guarantor:	prof. doc. Ing. Vladimír Havlíček, Dr.
Teacher(s):	prof. doc. Ing. Vladimír Havlíček, Dr.

Opinion survey results Examination dates SS schedule

Annotation -

Last update: doc. RNDr. Karel Nesměrák, Ph.D. (13.09.2018)

During the lectures, fundamentals, newest technologies and interpretation strategies will be reviewed. Basic knowledge acquisition will be guaranteed for students coming from diverse study fields. Ion formation mechanisms, dissociation techniques and product ion mass spectra will be discussed during the lectures. Intraoperative mass spectrometry, imaging mass spectrometry in medicine, multimodal imaging, ion mobility, MS3D techniques and other recent tools will be reviewed. Hands-on sessions with software tools (CycloBranch, mMass, etc.) will be organized.

Literature -

Last update: doc. RNDr. Karel Nesměrák, Ph.D. (13.09.2018)

Luptakova D, Pluhacek T, Petrik M, Novak J, Palyzova A, Sokolova L, Skriba A, Sediva B, Lemr K, Havlicek V. 2017. Non-invasive and invasive diagnoses of aspergillosis in a rat model by mass spectrometry. Scientific Reports 7:16523.

Luptakova D, Pluhacek T, Palyzova A, Prichystal J, Balog J, Lemr K, Juranek I, Havlicek V. 2017. Meet interesting abbreviations in clinical mass spectrometry: from compound classification by REIMS to multimodal and mass spectrometry imaging (MSI). Acta Virologica 61:353-360.

Novak J, Sokolova L, Lemr K, Pluhacek T, Palyzova A, Havlicek V. 2017. Batch-processing of imaging or liquid-chromatography mass spectrometry datasets and De Novo sequencing of polyketide siderophores. Biochimica Et Biophysica Acta-Proteins and Proteomics 1865:768-775.

Prichystal J, Schug KA, Lemr K, Novak J, Havlicek V. 2016. Structural Analysis of Natural Products. Analytical Chemistry 88:10338-10346.

Requirements to the exam - Czech

Last update: doc. RNDr. Karel Nesměrák, Ph.D. (22.02.2024)

Zkouší se vše, co bylo odpřednášeno, zkouška probíhá kombinovanou formou.

Syllabus -

Last update: doc. RNDr. Karel Nesměrák, Ph.D. (13.09.2018)

Common interpretation approaches for conventional and product ion mass spectra good for low molecular weight organics. Per analogiam method, spectra or biopolymers. Electrospray and matrix-assisted laser desorption/ionization mass spectrometry, desorption electrospray, rapid evaporation/ionization mass spectrometry. Accurate mass and extreme mass resolving power analyzers (ion cyclotron resonance Fourier transform mass spectrometry, orbitrap), ion mobility mass spectrometry. Miniaturization. Collision-induced dissociation, electron transfer dissociation, electron capture dissociation. Analysis of peptides and identification or proteins: sample prep, mass determination, deconvolution, peptide fragmentation, peptide mapping, post-translation modifications, protein conformation, molecular interactions, proteomics, database search, protein quantitation. MS3D approaches, hydrogen-deuterium exchange, chemical crosslinking. Surface-enhanced mass spectrometry. Analysis of nucleotides/nucleosides, saccharides, lipids. Mass spectrometry imaging in medicine. Metabolomics and proteomics in infectious and tumour disease diagnostics. Practical sessions: data collection on private samples (diploma thesis) on FTICR, TOF-TOF. Nuclear magnetic resonance spectroscopy and electron microscopy demonstrations.