Subjects

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Comparative Biochemistry of Parasites - laboratory course - MB160C17

Title:	Praktikum z biochemie parazitů
Czech title:	Praktikum z biochemie parazitů
Guaranteed by:	Department of Parasitology (31-161)
Faculty:	Faculty of Science
Actual:	from 2023
Semester:	summer
E-Credits:	2
Examination process:	summer s.:
Hours per week, examination:	summer s.:0/1, C [TS]
Capacity:	12
Min. number of students:	unlimited
4EU+:	no
Virtual mobility / capacity:	no
State of the course:	taught
Language:	Czech
Note:	enabled for web enrollment the course is taught as cyclical

Guarantor:	doc. RNDr. Ivan Hrdý, Ph.D.
Teacher(s):	doc. RNDr. Ivan Hrdý, Ph.D.
Class:	Horizontální laminární box 120 cm Biohazard laminární box 200 cm Stolní chlazená centrifuga univerzální se více rot Invertovaný mikroskop s fluorescenční kamerou Elektroforéza DNA horizontální Zdroj napětí na elektroforézu Pipetmany (sada) Termoblok Mikrocentrifuga stolní Mikrocentrifuga malá Dataprojektor Mikroskop badatelský
Co-requisite :	MB160P17

Opinion survey results Examination dates SS schedule

Annotation -

Last update: doc. RNDr. Ivan Hrdý, Ph.D. (25.10.2019)

The course demonstrates basic techniques used for preparation of protist parasites for biochemical experiments. It demonstrates cell homogenization and fractionation using differential and isopycnic gradient centrifugation, spectrophotometric determinations of enzyme activities and liquid chromatography methods of protein purification.

Literature -

Last update: doc. RNDr. Ivan Hrdý, Ph.D. (25.10.2019)

Relevant literature: M.P. Deutscher: Guide to Protein Purification. Academic Press 1990.

Detailed and up-to-date protocols will available for every projet during the course.

Requirements to the exam -

Last update: doc. RNDr. Ivan Hrdý, Ph.D. (25.10.2019)

Active participation on the course, mastering of the methods, calculation of kinetic and molecular parameters of the studied proteins, 100% attendance.

Syllabus -

Last update: doc. RNDr. Ivan Hrdý, Ph.D. (25.10.2019)

1. Cell homogenization methods

2. Methods of cell fractionation, isolation of organelles

3. Principles of protein separation techniques

4. Enzyme activity determinations, kinetic measurements

5. Protein purification