Biological Techniques - MB151P137E

• Title: Biological Techniques
• Czech title: Metodiky biologického výzkumu
• Guaranteed by: Department of Cell Biology (31-151)
• Faculty: Faculty of Science
• Actual: from 2023 to 2023
• Semester: summer
• E-Credits: 5
• Examination process: summer s.:combined
• Hours per week, examination: summer s.:3/2, C+Ex [HT]
• Capacity: unlimited
• Min. number of students: unlimited
• 4EU+: no
• Virtual mobility / capacity: no
• State of the course: not taught
• Language: English
• Note: enabled for web enrollment
• Guarantor: prof. RNDr. Jan Černý, Ph.D.

Annotation

Last update: doc. RNDr. František Půta, CSc. (06.02.2022)

The course Biological Techniques explain experimental approaches and methodologies of modern biology.
Seminal scientific discoveries from the first three biological lectures of the Science curriculum will be put in context
with the particular research approach. Biological Techniques expands understanding of the biological concepts
and facts in a practical way necessary for successful research career. The list of covered techniques will
correspond to the broad diversity of biological disciplines, with the emphasis to reach state of art achievements. All
lecturers are active scientists, specialists in a field they teach.

The course Biological Techniques forms the integral unit with three prerequisite courses (From molecules to
cells, From cells to organisms and On the Evolution and Ecology).

The course is built from topical blocks (see Syllabus) each of them consisting of lecture (3h) and workshop
focused on primary literature as a Q&A session (2h).

Literature

Last update: RNDr. Nataša Šebková, Ph.D. (31.05.2022)

1. Understanding Light Microscopy (RMS - Royal Microscopical Society) 1st Edition, ISBN-13: 978-0470973752, 2019
2. Introduction to Light Microscopy: Tips and Tricks for Beginners, 1st ed. 2019 Edition, ISBN-13: 978-3030053925, 2019
3. Proteomics for Biological Discovery, 2nd Edition, ISBN-13: 978-1118279243, 2019
4. Genome Editing and Engineering: From TALENs, ZFNs and CRISPRs to Molecular Surgery, 1st Edition, ISBN-13: 978-1107170377, 2018
5. Bioinformatics: A Practical Guide to the Analysis of Genes and Proteins, 4th Edition, 2002
6. Lecture notes
7. Pre-recorded lectures
8. Problem sets

Requirements to the exam

Last update: doc. RNDr. František Půta, CSc. (06.02.2022)

Final mark is based on the oral examination (67%) and results of tests taken during the course (33%). Oral examination takes place during the examination period and students must first obtain the evaluation for Q&A sessions, workshops and take-home exercises.

Syllabus

Last update: doc. RNDr. František Půta, CSc. (06.02.2022)

Introduction to biological research (ethics, observation and experimentation, hypothesis driven research, correct interpretation of the primary data) Seeing is believing - principles of visualisation techniques: limitations, types (AFM, SEM, TEM, light-based), applications, data mining approaches, quantitation, 3D, life imaging, whole-animal imaging, labelling techniques, image analysis Structural biology Computational biology Genetic analysis (sequencing, transcriptomics, epigenomics, study of polymorphisms) Bioinformatics Characterization of protein structure and function - proteomics, NMR, X-ray… Single cell techniques Gene modifications and phenogenomics Application of fluorescent proteins and probes Cladistics Flow cytometry and usage of antibodies Neurophysiological techniques