Last update: RNDr. Daniela Horníková, Ph.D. (24.03.2019)
1. Introduction to Transgenesis - Basic principles and strategies, transgenic organisms
2. Molecular genetics - Definition of gene function, mouse genetics and genetic mapping
3. Epigenetics. Imprinting and inactivation of chromosome X
4. Gametogenesis, fertilization, human and mouse stem cells
5. Transgenesis I. BAC transgenesis. Binary transgenic systems. Conditional targeting and homologous recombination. Recombination systems
6. Transgenesis II. Design of vectors for transgenesis. Electroporation and selection. Preparation of chimeras / transfer to germinal lines
7. Reporter mice and lineage tracing
8. Principles of mouse phenotyping. Pivotal mouse models and phenotypes
9. Modeling Human Diseases I. Muscular system
10. Modeling Human Diseases II. Gastrointestinal system
11. Modeling Human Diseases III. Nervous system, neurodegenerative diseases
12. Modeling Human Diseases IV. Cancer
13. The CCP phenotypic platform. Ethical and legal aspects of animal model-based research
Last update: RNDr. Daniela Horníková, Ph.D. (25.03.2019)
1. Introduction to Transgenesis - Basic principles and strategies, transgenic organisms
2. Molecular genetics - Definition of gene function, mouse genetics and genetic mapping
3. Epigenetics. Imprinting and inactivation of chromosome X
4. Gametogenesis, fertilization, human and mouse stem cells
5. Transgenesis I. BAC transgenesis. Binary transgenic systems. Conditional targeting and homologous recombination. Recombination systems
6. Transgenesis II. Design of vectors for transgenesis. Electroporation and selection. Preparation of chimeras / transfer to germinal lines
7. Reporter mice and lineage tracing
8. Principles of mouse phenotyping. Pivotal mouse models and phenotypes
9. Modeling Human Diseases I. Muscular system
10. Modeling Human Diseases II. Gastrointestinal system
11. Modeling Human Diseases III. Nervous system, neurodegenerative diseases
12. Modeling Human Diseases IV. Cancer
13. The CCP phenotypic platform. Ethical and legal aspects of animal model-based research