Subjects

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Practical course of diagnostic methods in virology - MB140C85

Title:	Praktikum z vybraných diagnostických metod ve virologii
Czech title:	Praktikum z vybraných diagnostických metod ve virologii
Guaranteed by:	Department of Genetics and Microbiology (31-140)
Faculty:	Faculty of Science
Actual:	from 2023 to 2023
Semester:	winter
E-Credits:	3
Examination process:	winter s.:
Hours per week, examination:	winter s.:0/1, C [TS]
Capacity:	12
Min. number of students:	6
4EU+:	no
Virtual mobility / capacity:	no
State of the course:	taught
Language:	Czech
Level:	specialized
Note:	enabled for web enrollment

Guarantor:	RNDr. Jana Šmahelová
Teacher(s):	RNDr. Ingrid Poláková, Ph.D. RNDr. Martina Saláková, Ph.D. RNDr. Jana Šmahelová
Co-requisite :	MB140P75
Incompatibility :	MB140C69E
Is incompatible with:	MB140C69E

Opinion survey results Examination dates WS schedule

Annotation -

Last update: RNDr. Ingrid Poláková, Ph.D. (22.08.2023)

This practical course will focus on molecular methods of viral detection in clinical specimens including the
interpretation of results. The methods will include extraction of nucleic acids from different type of clinical material. The students will analyze the quality and quantity of nucleic acids. They will also detect the selected genes by the polymerase
chain reaction (PCR) and/or real time PCR. The specificity of amplicons will be confirmed by sequencing.
For the detection of viral specific antibodies Enzyme-Linked Immunosorbent
Assay (ELISA) and/or indirect immunofluorescence method will be used.
The course will be held in BIOCEV, Vestec from Oct 23 to 27

Literature -

Last update: RNDr. Jana Šmahelová (06.09.2022)

See MB140P75

Requirements to the exam

Last update: RNDr. Ingrid Poláková, Ph.D. (22.08.2023)

The course will be held in BIOCEV, Vestec from Oct 23 to 27

In order to obtain credits the students have to provide the protocols per each group (2 students per group). The protocols have to include interpretation of the results.

Syllabus -

Last update: RNDr. Ingrid Poláková, Ph.D. (29.08.2022)

Direct methods - PCR, qRT PCR, sequencing

Indirect methods - imunofluorescence, ELISA

Emphasis will be on good clinical practice. The course will mimic the situation in the clinical laboratory.