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Molecular and cellular biology of model organism - MB140C78

Title:	Kurs práce s modelovým organismem
Czech title:	Kurs práce s modelovým organismem
Guaranteed by:	Department of Genetics and Microbiology (31-140)
Faculty:	Faculty of Science
Actual:	from 2022
Semester:	winter
E-Credits:	5
Examination process:	winter s.:
	summer s.:
Hours per week, examination:	winter s.:0/5, --- [HT] summer s.:0/5, C [HT]
Capacity:	11
Min. number of students:	unlimited
4EU+:	no
Virtual mobility / capacity:	no
State of the course:	taught
Language:	Czech
Level:	specialized

Guarantor:	prof. RNDr. Zdena Palková, CSc.
Teacher(s):	RNDr. Michal Čáp, Ph.D. Mgr. Martin Kuthan, Ph.D. prof. RNDr. Zdena Palková, CSc. RNDr. Michaela Schierová, Ph.D.

Opinion survey results Examination dates WS schedule SS schedule

Annotation -

Last update: RNDr. Irena Lichá, CSc. (26.05.2017)

Two-semester Oxford-type practical course is focused on work with model organism - the yeast Saccharomyces
cerevisiae. Each student will solve his own mini-project consisting of construction of a yeast strain carrying gene of
interest fused with green fluorescent protein or other fluorescent protein in the genome and subsequent analysis of
this strain. The course is designed as an introduction to laboratory work with a real project. At each stage of the
experimental work, students will learn the methods used for each step and subsequently will use the new know-
how to get familiar with individual steps of preparing their own genetically modified yeast strain. The course
capacity is limited by the complexity of the methods used, but the low number of course participants allows to
maximize individual approach and provides sufficient opportunities for discussion. Students are enrolled in the
course on the basis of interest assessment test.

Literature -

Last update: RNDr. Michal Čáp, Ph.D. (24.10.2019)

Study materials are provided by the teachers in the class.

Requirements to the exam -

Last update: RNDr. Michal Čáp, Ph.D. (24.10.2019)

Course credit requirements:

class attendance

results documentation

presentation of results at the end of the course

Syllabus -

Last update: RNDr. Michaela Schierová, Ph.D. (23.10.2019)

During the first part of the course, students will first learn basic laboratory tasks such as preparation and sterilization of solutions, buffers and microbiological media. It also introduces the basics of sterile work, inoculation and cultivation of microorganisms on solid and liquid media and monitoring of the growth curve.

In the second part of the course, students will learn the methods needed to work with DNA, such as plasmid DNA electroporation into bacteria, its growth and preparation of bacteria, and electrophoretic verification of isolated plasmid DNA including restriction analysis. Plasmid DNA thus obtained will be used as a template for in-vitro cloning of the transformation cartridge using PCR with primers according to its own design. The transformation cartridges will then be transformed into a yeast strain and the success of the prepared gene fusion will be verified again using PCR with primers according to their own design.

The third part of the course is devoted to analyzing prepared strains. Genetically modified yeast strains will be analyzed using a fluorescent microscope, spectrofluorimeter and flow cytometry. For these analyses will be used instrumentation in BIOCEVu in Vestci near Prague.