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Molecular biology and virology practical training course - MB140C77

Title:	Praktikum z molekulární biologie a virologie
Czech title:	Praktikum z molekulární biologie a virologie
Guaranteed by:	Department of Genetics and Microbiology (31-140)
Faculty:	Faculty of Science
Actual:	from 2022
Semester:	summer
E-Credits:	3
Examination process:	summer s.:
Hours per week, examination:	summer s.:0/1, C [TS]
Capacity:	18
Min. number of students:	5
4EU+:	no
Virtual mobility / capacity:	no
State of the course:	taught
Language:	Czech
Level:	specialized
Note:	enabled for web enrollment

Guarantor:	Mgr. Vojtěch Šroller, Ph.D.
Teacher(s):	Mgr. Martin Fraiberk, Ph.D. RNDr. Lenka Horníková, Ph.D. Boris Rjabčenko, Ph.D. Mgr. Vojtěch Šroller, Ph.D.
Class:	Horizontální laminární box 120 cm Biohazard laminární box 200 cm Stolní chlazená centrifuga univerzální se více rot Invertovaný mikroskop s fluorescenční kamerou Elektroforéza DNA horizontální Zdroj napětí na elektroforézu Pipetmany (sada) Termoblok Mikrocentrifuga stolní Mikrocentrifuga malá Dataprojektor Mikroskop badatelský

Opinion survey results Examination dates SS schedule

Annotation -

Last update: Mgr. Vojtěch Šroller, Ph.D. (08.01.2024)

One week course from molecular biology and virology provides both practical and theoretical training in laboratory
methods from molecular biology and virology. The selected experiments allow students to practice not only basic
methods (like DNA isolation, gel electrophoresis) but also methods more advances, which are used in biomedical
research and diagnostics laboratories (like electron microscopy, PCR, sequencing). Laboratory course is well
suited for students interested in molecular biology and virology.

Literature -

Last update: Mgr. Vojtěch Šroller, Ph.D. (25.10.2019)

Brown T.A. (2006, 2010) Gene Cloning and DNA Analysis: An Introduction; Willey-Blackwell

Sambrook J., Russell D.W., (2001) Molecular Cloning: A Laboratory Manual, third edition, Cold Spring Harbor Laboratory Press

Vondrejs V. (1997, 2001, 2003), Genové inženýrství I-III, Karolinum

Requirements to the exam -

Last update: Mgr. Vojtěch Šroller, Ph.D. (30.08.2019)

credit based on written protocols

Syllabus -

Last update: RNDr. Irena Lichá, CSc. (26.05.2017)

The work schedule of practical course:

1. Isolation of DNA, RNA and proteins from mouse cell line, measuring concentration and purity (spectrophotometry)

2. Isolation of bacterial plasmid DNA, visualization on gel electrophoresis, restriction analysis, methods for cloning of genes.

3. Detection of virus specific antibodies in pigs’ sera, using hemagglutination and neutralization inhibition test.

4. Visualization of pseudovirions by electron microscopy.

5. Isolation and detection viral DNA from skin using methods rolling cycle amplification, PCR multiplex amplification, sequencing and identification of DNA by computer analysis.