Subjects

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Molecular Cloning - MB140C38

Title:	Molecular Cloning
Czech title:	Klonování na úrovni genů
Guaranteed by:	Department of Genetics and Microbiology (31-140)
Faculty:	Faculty of Science
Actual:	from 2023
Semester:	summer
E-Credits:	3
Examination process:	summer s.:
Hours per week, examination:	summer s.:0/4, C [DS]
Capacity:	12
Min. number of students:	6
4EU+:	no
Virtual mobility / capacity:	no
State of the course:	taught
Language:	English
Level:	specialized
Note:	enabled for web enrollment

Guarantor:	Mgr. Václav Vopálenský, Ph.D.
Teacher(s):	RNDr. Tomáš Mašek, Ph.D. Mgr. Kristina Roučová Mgr. Václav Vopálenský, Ph.D.
Class:	Ultracentrifuga a 3 rotory Gradientový PCR cycler Původní předmět

Opinion survey results Examination dates SS schedule

Annotation -

Last update: RNDr. Irena Lichá, CSc. (14.05.2021)

In this three-day practical course, participants will learn basic molecular cloning techniques from in silico cloning
strategy to the practical molecular cloning of the selected gene into a plasmid-type expression vector with
subsequent verification of the construct using classical molecular biological methods (PCR, restriction
endonuclease digestion or sequencing). Then the course participants will try to express the selected protein in a
bacterial expression system. This part of the practical course includes the electroporation of the constructed
plasmid into the appropriate bacterial strain and the subsequent induction of the expression system to produce
the selected protein, including the subsequent analysis of protein production by PAAGE electrophoresis.