Experimental Pharmacodynamics - GAF372

• Title: Experimental Pharmacodynamics
• Guaranteed by: Department of Pharmacology and Toxicology (16-16170)
• Faculty: Faculty of Pharmacy in Hradec Králové
• Actual: from 2021
• Semester: summer
• Points: 0
• E-Credits: 3
• Examination process: summer s.:oral
• Hours per week, examination: summer s.:14/28, C+Ex [HS]
• Capacity: unknown / unknown (16)
• Min. number of students: unlimited
• 4EU+: no
• Virtual mobility / capacity: no
• State of the course: taught
• Language: English
• Teaching methods: full-time
• Teaching methods: full-time
• Is provided by: GF372
• Note: deregister from the exam date if a requisite was not fulfilled; course can be enrolled in outside the study plan; enabled for web enrollment
• Guarantor: PharmDr. Alejandro Carazo Fernández, Ph.D.
• Pre-requisite : GAF339

Annotation

Last update: PharmDr. Alejandro Carazo Fernández, Ph.D. (14.02.2024)

The purpose of this subject is to provide a complete background in the area of experimental pharmacodynamics. The students will be theoretically and practically trained in the basic principles of pharmacodynamic experimentation including data elaboration and statistical analysis. The subject is taught fully and only in English. Conditions: • This subject is an elective subject in the summer term of 4th year of the study. Is it possible to register the subject only after passing Pharmacology II and Pharmaceutical Chemistry II. • Exam is oral in English, credits will be awarded based on at least 4 from 7 attendances on practical classes (all students must attend practical class No.1, and choose 3 additional practical classes) • The student will obtain 3 credits after successfully completing the subject. Content: The subject is composed from 7 practical classes (4 hours each, 28 in total) and 7 lectures (2 hours each, 14 hours in total) which will precede and match with the topics of the practical classes. Maximal capacity: 16 students (4 groups of 4 students)

Course completion requirements

Last update: PharmDr. Alejandro Carazo Fernández, Ph.D. (14.02.2024)

The student will select at least 3 from a total of 6 practical classes (No.2-7) and must previously attend the corresponding lecture. Attendance of the 1st practical class and presentation is compulsory.

After successful compliance with the requirements, the students will take a brief oral exam that will assert the knowledge on the topics discussed in the subject, particularly on the laboratory methods employed

Literature

Last update: PharmDr. Alejandro Carazo Fernández, Ph.D. (14.02.2024)

Recommended:

• Ritter J.M., Flower R. et al.. Rang and Dale´s Pharmacology. : Elsevier, 2020, s. ISBN 978-0-7020-7448-6.
• Hardman, Joel G. Limbird, Lee E. Gilman, Alfred Goodman, (eds.). Goodman & Gilman's the pharmacological basis of therapeutics. New York: McGraw-Hill, Medical Publishing Division, 2001, 2148 s. ISBN 0-07-135469-7.

Syllabus

Last update: PharmDr. Alejandro Carazo Fernández, Ph.D. (14.02.2024)

Lectures (L):

1.       Physiology, pathology and pharmacology of trace elements (iron, copper and zinc) 

2.       Experimental determination of metal chelation, and metal based anti- or prooxidant properties 

3.       Physiology of blood coagulation and its testing 

4.       Cardiac toxicity 

5.       Intracellular receptors 

6.       Platelets, their structure, receptors, pathways and experimental results 

7.       Experimental testing of the effects of compounds on vascular smooth muscles 

Practical lectures (PL):

1.       Basic laboratory skills (weighting, pipetting, preparation of a calibration curve) – Mandatory

2.       Screening of metal chelation activity 

3.       Screening of anticoagulant activity 

4.       Use of cell culture models for the study of toxic effects of drugs 

5.       Drug effects on intracellular receptors 

6.       Testing of potentially novel drugs at the platelet aggregation level 

7.       Testing of the effect of potentially novel drugs on the level of vascular smooth muscles