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Phytochemical Methods - GAF226

Title:	Phytochemical Methods
Guaranteed by:	Department of Pharmacognosy and Pharmaceutical Botany (16-16230)
Faculty:	Faculty of Pharmacy in Hradec Králové
Actual:	from 2023
Semester:	winter
Points:	0
E-Credits:	2
Examination process:	winter s.:written
Hours per week, examination:	winter s.:14/28, Ex [HS]
Capacity:	12 / 12 (12)
Min. number of students:	unlimited
4EU+:	no
Virtual mobility / capacity:	no
Key competences:
State of the course:	taught
Language:	English
Teaching methods:	full-time
Teaching methods:	full-time
Level:
Explanation:	(F 3.r.)
Note:	course can be enrolled in outside the study plan enabled for web enrollment

Guarantor:	prof. Ing. Lucie Cahlíková, Ph.D.

Examination dates WS schedule

Annotation -

Last update: prof. Ing. Lucie Cahlíková, Ph.D. (08.11.2021)

In this subject the students familiarize with the special isolation and analytical procedures, which are involved in the work with plant material for the effective isolation of compounds of different types and for determination of content of secondary metabolites by the modern analytical methods. In the practical classes the students familiarize with the procedure of processing of row material which leads to the identification of compounds. The education is directed to creative approach during the isolation of compounds and phytochemical analyses. The subject is intended for applicants who are interested in the diploma thesis with phytochemical theme.

Literature -

Last update: prof. Ing. Lucie Cahlíková, Ph.D. (08.11.2021)

Obligatory:

. . In Harborne, J. B.. Phytochemical methods : a guide to modern techniques of plant analysis . London: Chapman & Hall, 1998, s. -. ISBN 0-412-57270-2..

Syllabus -

Last update: prof. Ing. Lucie Cahlíková, Ph.D. (08.11.2021)

Thematic areas of lectures

1. Introduction to phytochemical methods.

2. Methods of extraction and isolation

3. Chromatographic methods (TLC, prep. TLC, HPLC, HPLC/ MS, GC, GC/MS ) I

4. Chromatographic methods (TLC, prep. TLC, HPLC, HPLC/ MS, GC, GC/MS ) II

5. Spectroscopic methods for identification of secondary metabolites (IR, NMR, MS)

6. Overwiev of secondary metabolites

Lectures are held every odd Wednesday 8:50 – 10:20 a.m. in the seminary room in the Garden of medicinal plants.

Practical classes

Preparation of capillary, Pasteur pipete, pen for preparative thin layer chromatography

Thin layer chromatography: identification of unknown compound

Preparative thin layer chromatography

Preparation of alkaloid extract from plant

Flash chromatogryphy

GC/MS analysis of alkaloid extract and identification of alkaloids

GC-FID identification of galanthamine, lycorine and haemanthamine in extracts

Preparative HPLC+ HPLC analysis of herbal tea

Entry requirements -

Last update: prof. Ing. Lucie Cahlíková, Ph.D. (08.11.2021)

Learned knowledges from pharmaceutical botany, organic chemistry, bioorganic chemistry and basic knowledges from analysis of organic compounds.

Teaching methods -

Last update: prof. Ing. Lucie Cahlíková, Ph.D. (08.11.2021)

Lectures, practical classes.

Requirements to the exam -

Last update: prof. Ing. Lucie Cahlíková, Ph.D. (08.11.2021)

The credit is given on the basis of attendance and the practical abilities of preparation of basic glass equipment, which could be prepared in the laboratory (capillary tube for sample application in TLC, Pasteur pipette, glass ball for transfer of liquids and their filtration, crystallization vials according to Svoboda)
The exam is written test with 15 questions, one of them is interpretive (solution of example of optimal natural substances mixture separation).