Cell Lines in Oncology Treatment Research - EDPLB0021

• Title: Cell Lines in Oncology Treatment Research
• Guaranteed by: Department of science and research (14-260)
• Faculty: Faculty of Medicine in Pilsen
• Actual: from 2023
• Semester: winter
• Points: 0
• E-Credits: 0
• Examination process: winter s.:
• Hours per week, examination: winter s.:3/3, C [HT]
• Capacity: unknown / unknown (unknown)
• Min. number of students: unlimited
• 4EU+: no
• Virtual mobility / capacity: no
• State of the course: taught
• Language: English
• Teaching methods: full-time
• Teaching methods: full-time
• Guarantor: doc. RNDr. Martin Pešta, Ph.D.

Annotation

Last update: Mgr. Barbora Stachová (06.05.2024)

The aim of the course is to familiarize students with the methods of cultivation of cell lines and their analysis at the
level of genotype and phenotype when investigating the effect of molecules affecting molecular biological and
biochemical processes in the cell.
This will be explained using the example of research on molecules for targeted cancer therapy and classical
chemotherapy. Most of the procedures presented have general applicability to research using cell line models.

Literature

Last update: Mgr. Barbora Stachová (06.05.2024)

M. Aschner, Cell Culture Techniques, ISBN 978-1-61779-076-8, Springer Science+Business Media

V. Liška et al.: Experimental Surgery, 2016, ISBN 978-80-7211-490-0, NAVA

Syllabus

Last update: Mgr. Barbora Stachová (06.05.2024)

Cell culture is now an indispensable model for both basic and applied research. After a brief historical introduction, the types of cell cultures will be explained. The types of media and the purpose of their use will then be described, as well as basic equipment for tissue culture laboratories. Examples of the application of cell culture in research with emphasis on oncology will be presented. Cultivation of tumor cells will be described with an extensive table of selected tumor cell lines from the American Type Culture Collection, Rockville, Maryland, U.S.A. Specific examples of cancer therapy research and methods for assessing genotypic and phenotypic characteristics of cell lines, including methods of protein and gene expression analysis will be presented. Upon completion of the course, students will be able to perform basic experimental design to affect the given properties of cell lines.