The elective subject Molecular Imaging Methods provides an overview of the modern method of structural and
molecular imaging of human body tissues by imaging methods with a focus on internal imaging
structure, chemical composition of tissues, blood circulation of tissues, exchange of substances between individual compartments by methods
computed tomography and magnetic resonance imaging, as well as hybrid imaging with positron emission
tomography. The course provides an overview of clinical applications of various types of molecular imaging and their
clinical perspective in the future.
Course completion requirements
Last update: Ing. Lenka Křikavová (03.01.2023)
online test in the Moodle course
Literature - Czech
Last update: Ing. Lenka Křikavová (18.05.2021)
Povinná literatura:
Ferda J, Mírka H, Baxa J, Malán A. Základy zobrazovacích metod, Galén, Praha, 1. vydání, 2015
Doporučená literatura:
Ferda J, Kreuzberg B, Novák M. Výpočetní tomografie, Galén, Praha, 1. vydání 2002
1. Basic methods and procedures of molecular imaging : Indicator principle, distribution of contrast agents, perfusion imaging, magnetic resonance spectroscopy, microstructure imaging by diffusion weighing in magnetic resonance imaging, use of radiopharmaceuticals
2. Densitometry using CT: Hounsfield scale, densitometry of tissues, imaging using CT with dual energy, chemical analysis using CT
4. Perfusion display using CT: perfusion parameters - Patlak's model - mean transit time, blood volume, blood perfusion, time to peak
5. Perfusion imaging using MRI: Perfusion parameters - Tofts model - iAUC, Ktrans, Ve, elimination constant, T2* magnetic resonance perfusion retardation, arterial spin labeling
6. Imaging of the microstructure of tissues by diffusion weighted magnetic resonance imaging: The principle of diffusion weighing, diffusion restriction, diffusion facilitation, apparent diffusion coefficient, fractional anisotropy, intracellular oedema retardation, tissue cellularity imaging, tractography
7. Magnetic resonance imaging of tissue chemical composition: Hemoglobin decay products, melanin, fat, calcium, susceptible weighing, in-phase/opposed-phase, Dixon imaging, water/fat imaging
8. Magnetic resonance spectroscopy: chemical shift, magnetic resonance and spectroscopy, hydrogen spectroscopy, phosphor spectroscopy, brain tissue spectroscopy, prostate spectroscopy, breast tumor spectroscopy
9. Magnetic resonance relaxometry: Principle of relaxometry using magnetic resonance imaging, analysis of cartilage composition, analysis of myocardial structure
10. Imaging of energy metabolism of tissues: Glycolysis of tissues and its imaging, normal differences in glycolytic activity of tissues, changes in glycolytic activity in tumor tissue, Warburg phenomenon, tissues and glycolysis, positron emission tomography and utilization of 18f-fluorodeoxylucose
11. Tissue construction and proliferative activity of tissues: proteosynthesis of tissues and its imaging with 18F-fluoroethyltyrosine, construction of cell membranes and its imaging with 18F-fluorocholine, synthesis of nucleic acids and its imaging with 18F-fluorothymidine, bone restoration and its resolution with 18F-fluorothymidine markers
12. Receptor imaging: Receptor principle of imaging, receptor imaging in dopaminergic disorders of the brain using PET and SPECT, receptor imaging of somatostatin receptors (111In-octreotide, 68Ga-DOTA-derivatives), receptor imaging using protein ligands (68Ga labeled ligands PSMA), receptor principle therapy with beta minus emitter (177Lu, 90Y)
