|
|
|
||
|
Last update: Lenka Kostohryzová (04.08.2023)
|
|
||
|
Last update: RNDr. Petr Heřman (02.11.2017)
No exam |
|
||
|
Last update: Mgr. Jana Čechová (08.09.2019)
Introduction to tissue engineering • Tissue structure – overview • Selected techniques and methods applied in tissue and protein engineering – summary (lasers, fluorescence, flow cytometry, FACS, electromigration and chromatographic methods, PCR, spectroscopic and spectrometric methods) • Regenerative medicine and tissue engineering • Cells in tissue engineering – principle sources, cells isolated from tissues, embryonic and adult stem cells
Tissue engineering • Cartilage tissue engineering • Bone tissue engineering • Skin covers
Nanofibers, their production and safety • Nanofibers and production methods • Safety of nanofibers and other nanosystems • Medical textiles as scaffolds for cell seeding • Reasons and methods of nanofiber functionalization
Bio-nanotechnology and systems of controlled drug release • Application of bio-nanotechnology in tissue engineering and regenerative medicine • Controlled drug delivery, liposomes, their preparation and application • „Smart nanofiber/liposome systems“ for controlled delivery of bioactive molecules
Bio-nanotechnology for artificial tissue production • Exogenous and endogenous cell sources • Bio-nanotechnology and mobilization of endogenous cell sources • Biomechanical properties on native and artificial tissues and their determination • Quality testing of bio-implants (in vitro and in vivo tests)
Practicals– UCEEB CTU Martin Královič martin_kralovic@labdemo.cz Univerzitní centrum energeticky efektivních budov ČVUT v Praze Laboratoř pokročilých biomateriálů Třinecká 1024 273 43 Buštěhrad
A. Nanofibers and controlled drug delivery (UCEEB CTU) Task 1: Preparation of nanofibers by electrospinning and forcespinning methods Task 2: Visualization of nanofiber's morphology (SEM analyses) Task 3: Demonstration of 3D printing method Task 4: Evaluation of physico-chemical properties of nanofibres (FTIR analysis)
B. In vitro characterization of cells Institute of Experimental Medicine of the ASCR (IEM ASCR) eva.filova@iem.cas.cz Institute of Experimental medicine of the ASCR IBC building Vídeňská 1083 142 20 Praha 4
Task 1: Cell passaging Task 2: Cell staining, confocal microscopy – DiOC6 and propidium iodide staining Task 3: Cells metabolic activity testing – MTS test
|