OS - Stem cells and gene manipulation in regenerative medicine - DVA1089

• Title: OS - Stem cells and gene manipulation in regenerative medicine
• Guaranteed by: Department of Neuroscience (13-610)
• Faculty: Second Faculty of Medicine
• Actual: from 2021
• Semester: summer
• Points: 3
• E-Credits: 3
• Examination process: summer s.:
• Hours per week, examination: summer s.:0/20, C [HT]
• Capacity: unknown / unknown (12)
• Min. number of students: 3
• 4EU+: no
• Virtual mobility / capacity: no
• State of the course: not taught
• Language: English
• Teaching methods: full-time
• Teaching methods: full-time
• Note: course can be enrolled in outside the study plan; enabled for web enrollment; priority enrollment if the course is part of the study plan
• Guarantor: doc. MUDr. Lýdia Vargová, Ph.D.; doc. RNDr. Pavla Jendelová, Ph.D.

Annotation

Last update: PROCH (05.10.2015)

Embryonální, fetální, mezenchymální a tkáňově specifické kmenové buňky; teoretické základy, vývoj v oboru. Praktické aplikace kmenových buněk v terapii závažných onemocnění.

Course completion requirements

English

Last update: Mgr. Jana Čechová (13.09.2018)

80% prezence in OS

Requirements to the exam

Last update: Mgr. Jana Čechová (10.02.2019)

Discussion with scholar on the main topics of OS.

Syllabus

English

Last update: Mgr. Jana Čechová (13.09.2018)

Sylabus:
Day 1 Monday: Department of Neuroscience 2.LF UK Plzeňská 130/221, Prague 5 - Motol

Lectures: Introduction to stem cell biology and biomaterials (Jendelová), Safety, laboratory work (Růžička)

Practical course: NA

Day 2 Tuesday: Department of Neuroscience 2.LF UK Plzeňská 130/221, Prague 5 - Motol

Lectures: Embryonic stem cells and induced pluripotent cells, Neural stem cells: their isolation, characterization and use in experiments (Jiráková, Jendelová)

Practical course: seminar - MSC isolation from rat bone marrow (femur, tibia), preparation of primary cultures (video with comment, 15 min), - Implantation of hydrogels into a hemisection model of SCI (video with comment, 30 min), - Balloon compression lesion model of SCI (video with comment, 15min), - Behavioural tests to evaluate motor and sensory function in a rat model of SCI (video with comment, 20 min

Day 3 Wednesday: Institute of Experimental Medicine ASCR. Videnska 1083, Prague 4, Building LA

Lectures : NA

Practical session (Růžička): Work in a flow box, preparation of materials for tissue engineering (nanofibers, hydrogels, hydroxyapatite), seeding of MSCs - Cell culture (media exchange, passaging, cell counting, basic microscopy), Checking of confluence, Passaging, cell counting, Seeding stem cells on materials (10min)

Day 4: Thursday Department of Neuroscience 2.LF UK

Plzeňská 130/221, Prague 5 - Motol

Hematopoetic stem cells and mesenchymal stem cells from bone marrow, adipose tissue and Wharton Jelly and their use in regenerative medicine (Vacková), Cell labeling with iron-oxide nanoparticles and their monitoring by MR imaging. (Jendelová)

Practical session (Růžička) -Microscopy -Analysis of cell survival in spinal cord injury, -Image analysis software - Image J (cell counting, area summation), Axiovision Wizard software, Image analysis - hydrogel properties, cell attachment (10 min), Nanofibers - idea of orientation Hydrogels - surface characteristics, porosity, modifications, Confocal vs. fluorescence microscopy

Day 5, Friday: Department of Neuroscience 2.LF UK Plzeňská 130/221, Prague 5 - Motol

Advance therapy medicinal products (ATMP) in clinical and preclinical studies (Kubinová)