Detection methods of DNA, RNA and proteins in medicine - CVOL68

• Title: Detection methods of DNA, RNA and proteins in medicine
• Guaranteed by: Department of Biochemistry, Cell and Molecular Biology 3FM CU (12-BIOC)
• Faculty: Third Faculty of Medicine
• Actual: from 2023
• Semester: summer
• Points: 3
• E-Credits: 3
• Examination process: summer s.:
• Hours per week, examination: summer s.:0/20, C [HS]
• Capacity: unknown / unknown (10)
• Min. number of students: 2
• 4EU+: no
• Virtual mobility / capacity: no
• State of the course: taught
• Language: English
• Teaching methods: full-time
• Teaching methods: full-time
• Note: course can be enrolled in outside the study plan; enabled for web enrollment
• Guarantor: RNDr. Kamila Balušíková, Ph.D.; RNDr. Vlasta Němcová, Ph.D.
• Comes under: AVSEOB-VP-3 (ZÁPIS); AVSEOB-VP-4 (ZÁPIS); AVSEOB-VP-5 (ZÁPIS); AVSEOB-VP-6 (ZÁPIS); AVSEOB1-VP-3 (ZÁPIS)
• Pre-requisite : CVSE2P0040

Course completion requirements

Last update: RNDr. Kamila Balušíková, Ph.D. (29.09.2017)

 

 

Participation at least during 80% of the course is essential for practice fulfillment.

 

In the case of non-fulfillment of the credit conditions, the student can obtain a credit on the basis of a seminar work. Specifics of the seminar work are discussed with teacher individually. If the student could not attend the class, for example due to a disease, a medical report can be accepted instead of the student’s attendance.

 

 

 

Teaching methods

Last update: RNDr. Kamila Balušíková, Ph.D. (29.09.2017)

The study is performed in the form of practices

Syllabus

Last update: RNDr. Kamila Balušíková, Ph.D. (29.09.2017)

A) Analysis of nucleic acids:

1) DNA and RNA isolation

2) Measurement of DNA and RNA concentration

3) PCR

4) Methods of mutations detection (RFLP, PCR with allele specific primers)

5) Electrophoresis of DNA and RNA

6) Real-time PCR*

7) Detection of microRNA*

8) DNA sequencing*

 

B) Analysis of proteins:

1) Proteins isolation

2) Measurement of proteins concentration

3) Electrophoresis of proteins (SDS-PAGE)

4) Western blot analysis

5) Fluorescent microscopy

6) ELISA*

7) Flow cytometry*

 

*only theoretical lessons

 

 

Course completion requirements

Last update: RNDr. Kamila Balušíková, Ph.D. (29.09.2017)

 

 

Participation at least during 80% of the course is essential for practice fulfillment.

 

In the case of non-fulfillment of the credit conditions, the student can obtain a credit on the basis of a seminar work. Specifics of the seminar work are discussed with teacher individually. If the student could not attend the class, for example due to a disease, a medical report can be accepted instead of the student’s attendance.