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Course, academic year 2023/2024
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Advanced methods in microscopy - CCOCA0090
Title: Advanced methods in microscopy
Guaranteed by: Department of Histology and Embryology 3FM CU (12-HIST)
Faculty: Third Faculty of Medicine
Actual: from 2020
Semester: summer
Points: 1
E-Credits: 1
Examination process: summer s.:
Hours per week, examination: summer s.:0/15, C [HS]
Capacity: unknown / unknown (10)
Min. number of students: 3
4EU+: no
Virtual mobility / capacity: no
Key competences:  
State of the course: not taught
Language: English, Czech
Teaching methods: full-time
Teaching methods: full-time
Level:  
Note: course can be enrolled in outside the study plan
enabled for web enrollment
Guarantor: RNDr. Radek Pelc, D.Phil.
Pre-requisite : CVSE1M0001
Examination dates   Schedule   
Annotation -
Last update: RNDr. Radek Pelc, D.Phil. (12.12.2019)
Special histological procedures. Special microscopy modalities. History of staining protocols (textile and cosmetic dyes versus staining of biological tissue in medicine).
Aim of the course -
Last update: RNDr. Radek Pelc, D.Phil. (12.12.2019)

see OUTLINE ("ANOTACE")

Course completion requirements -
Last update: RNDr. Radek Pelc, D.Phil. (12.12.2019)

Being present at seminars

Literature -
Last update: RNDr. Radek Pelc, D.Phil. (12.12.2019)

IN-DEPTH BIOLOGICAL MICROSCOPY:

Inoué S. & Spring K. (1997)
Video microscopy (2nd ed.).
Springer, New York.
ISBN: 978-0-306-45531-5
https://www.springer.com/gp/book/9780306455315

(may be borrowed from the teacher = Pelc R.)

 

SELECTION OF RECENTLY DEVELOPED APPROACHES IN HISTOLOGY AND MICROSCOPY:

Pelc R., Walz W. & Doucette J.R. (2020)

Neurohistology and imaging techniques.

Springer (Humana Press), New York

ISBN: 978-1-07-160426-7

https://www.springer.com/gp/book/9781071604267

(may be borrowed from the teacher = Pelc R.)

Syllabus -
Last update: RNDr. Radek Pelc, D.Phil. (12.12.2019)

- Immunohistochemistry

- EdU Click Kit (cell proliferation)

- Fluorescent labels (Fura-2, ...) a proteins (GFP, YFP, RFP, ...)

- Modulation contrast

- Ultramicroscopy (light-sheet microscopy)

- Fluorescence, confocal a two-photon microscopy

- Chemical (IR/Raman) microscopy (microspectroscopy)

- Digital photomicrography

Course completion requirements -
Last update: RNDr. Radek Pelc, D.Phil. (12.12.2019)

Being present at seminars

 
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