Practical Course of DNA diagnostics - B81680 (elective subject)

• Title: Praktický kurz DNA diagnostiky
• Guaranteed by: Department of Pediatrics and Inherited Metabolic Disorders First Faculty of Medicine Charles University and General University Hospital in Prague (11-00650)
• Faculty: First Faculty of Medicine
• Actual: from 2023
• Semester: winter
• Points: 2
• E-Credits: 2
• Examination process: winter s.:
• Hours per week, examination: winter s.:0/12, C [HS]
• Extent per academic year: 12 [hours]
• Capacity: 6
• Min. number of students: 2
• 4EU+: no
• Virtual mobility / capacity: no
• State of the course: taught
• Language: English
• Teaching methods: full-time
• Teaching methods: full-time
• Explanation: [e-mail]
• Note: course can be enrolled in outside the study plan; enabled for web enrollment
• Guarantor: RNDr. Hana Hansíková, CSc.; Ing. Markéta Tesařová, Ph.D.
• Attributes: Lékařství; Teoretický předmět; Zubní lékařství

Annotation

Last update: Marcela Holanová (29.04.2021)

Practical Course of DNA diagnostics Annotation: The aim of this practical course is to introduce recent basic techniques used in DNA diagnostics. After theoretical opening all students will have the possibility to try practically basic methods in lab. Course is designed for highly motivated students of 3rd to 6th year of general medicine studies, who are thinking about PhD studies; and is open also for PhD. students. Course is open for 6 students; takes 5-6 hours of two afternoons, and takes place in Laboratoř pro studium mitochondrialnich poruch, at the Dep. of Pediatrics (Klinika dětského a dorostového lékařství), Ke Karlovu 2, Praha 2, building E4. Notice: The exact date is arranged individually due to the signed students’ possibilities. Attendants please contact Iveta Tothova, PhD. by e-mail: [e-mail] (contact phone No. 224967748).

Syllabus

Last update: Marcela Holanová (29.04.2021)

Syllabus:

Practical course of DNA diagnostics.



Scope of course: theoretical opening; DNA isolation (classical methods and commercial kits) from different tissues (blood, hair, buccal cells, cultivated fibroblasts), concentration measurement (NanoDrop), methods of DNA amplification (PCR- practically; cloning - theoretically), mutation detection methods - implications and applications (practically: ELFO, RFLP; theoretically: sequencing, HA, DGGE). Molecular results interpretation.



Your questions, please, address to: [e-mail] or call 22496 7748.

Teaching methods

Last update: Ing. Markéta Tesařová, Ph.D. (23.10.2023)

Date: 14. - 15.12.2023 (12:30 - 18:00) 

Place: Laborator pro studium mitochondriálních poruch, which is part of the Department of Pediatrics anf Inherited Metabolic Disorders (Ke
Karlovu 2), in building E4. The entrance is from the right side of the building. Ring the bell "Mitolab Prijem
materialu" and come to the third floor.
Please, takeyour lab coat with you.