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Biochemistry and Pathobiochemistry 1 - B81129
Title: Biochemistry and Pathobiochemistry 1
Guaranteed by: Institute of Medical Biochemistry and Laboratory Diagnostics First Faculty of Medicine Charles University and General University Hospital in Prague (11-00410)
Faculty: First Faculty of Medicine
Actual: from 2023
Semester: summer
Points: 5
E-Credits: 5
Examination process: summer s.:
Hours per week, examination: summer s.:1/3, C [HT]
Extent per academic year: 60 [hours]
Capacity: unlimited
Min. number of students: unlimited
4EU+: no
Virtual mobility / capacity: no
State of the course: taught
Language: English
Teaching methods: full-time
Teaching methods: full-time
Explanation: Doc.MUDr. Ivan Malbohan,for info see web pages of institute
Additional information: http://ulbld.lf1.cuni.cz/
Guarantor: prof. MUDr. Tomáš Zima, DrSc., MBA
Attributes: Teoretický předmět
Zubní lékařství
Is pre-requisite for: B81150, B81138, B81153, B83113, B81139, B81144, B81148
Annotation -
Last update: Vladěna Topičová (22.09.2007)
Biochemistry and pathobiochemistry for dentists will be concentrated on the fundamentals of metabolism related to the most important and most frequent diseases which may be encountered by the dentist (e.g. hemocoagulation disturbances, diabetes etc.). In detail will be discussed especially the digestion processes, defence against infection (inflammation) and metabolism of connective and hard tissues (bone and tooth). The metabolism of calcium and its disturbances will be especially stressed. The biochemistry and pathobiochemistry of oral cavity (salive, dental plaque) will be stressed too.
Syllabus
Last update: Jana Kolářová (31.01.2020)
Lectures

General features of metabolism. Bioenergetics.

Digestion, absorption, storage and transport of saccharides.

The citric acid cycle.

Regulation of metabolism of saccharides.

Biomembranes. Composed lipids.

Lipoproteins: Classification, properties and metabolism.

Steroids: Synthesis and metabolism. Steroid hormones. Bile acids.

Conversion of amino acids to biologically active derivatives.

The connective tissue, bone and cartilage. Proteoglycans, collagen and elastin - metabolism.

Saliva.

Seminars

Biologic oxidation, mitochondrial respiratory chain and oxidative phosphorylation

Stereochemistry of saccharides

Metabolism of saccharides.

Overview of basic methods used in biochemical lab: photometry, chromatography, electrophoresis, blotting.

Lipids: Biosynthesis and degradation of fatty acids

Relationships between metabolism of lipids and saccharides. Starvation

Metabolism of amino acids: Transamination, deamination, synthesis of urea.

Porphyrins and bile pigments

Metabolism of calcium, phosphate, and other elements and vitamins necessary for mineralisation of bone and teeth.

Tooth: Chemical composition and structure

Practical lessons

Saccharides I: Colored reactions of saccharides. Analysis of unknown saccharide.

Saccharides II: Acidic hydrolysis of starch. Substrate specificity of amylase and sucrase. Polarimetry of glucose and fructose.

Introduction to spectrophotometry: Absorption spectra of colored solutions. Calibration curve, factor calculation.

Saccharides III: Estimation of glycemia. Glucose in urine.

Saliva: Demonstration of selected components

Lipids: Estimation of total cholesterol and triacylglycerols. Demonstration of double bonds in fatty acids. Test for malondialdehyde. Ketone bodies in urine.

Liver tests: Direct and indirect bilirubin. Estimation of aminotransferases.

Bone and teeth: Estimation of calcium and phosphate in serum and urine. Isoenzymes of alkaline phosphatase. Properties of dental hydroxyapatite.

Literature - Czech
Last update: MUDr. Lenka Fialová, CSc. (26.09.2019)

P.C. Champe, R.A. Harvey, and D.R. Ferrier: Lippincott s Illustrated Reviews: Biochemistry. Lippincott Williams & Wilkins (latest edition)

B. Alberts et al.: Essential Cell Biology. Garland Science Publishing, New York (latest edition)

R.K. Murray et al.: Harper?s Illustrated Biochemistry. McGraw-Hill, Inc., USA (latest edition)
Course completion requirements
Last update: MUDr. Lenka Fialová, CSc. (16.05.2019)

Credit requirements: 

  1. Sufficient attendance to the seminars and practical lessons. At most three seminars or practical lessons (altogether) are allowed to be missed in one term from serious and certifiable reasons.

  2. Passing the Revision test on the core metabolic pathways (announced separately). Two more chances to retake the test can be provided.

  3. Completed, handed in, marked and approved reports from the practical lessons.

  4. Passing the Practical Credit Examination (announced separately). Two more chances to retake the practical examination can be provided.

  5. Good knowledge of the subject matters covered by the course.
Education plan -
Schedule block
Week of the block Day From - To Education type Theme Teacher Files Note Hodnocení
1Tuesday13:30 - 16:00nothingAnatomical Dissections  
2Tuesday13:30 - 16:00nothingAnatomical Dissections  
3Tuesday13:30 - 15:00seminarBiologic oxidation, mitochondrial respiratory chain and oxidative phosphorylation MUDr. Jan Pláteník, Ph.D. 
4Tuesday13:30 - 15:00seminarStereochemistry of saccharidesMUDr. Jan Pláteník, Ph.D. 
Friday14:15 - 16:30practical classesSaccharides I: Colored reactions of saccharides. Analysis of unknown saccharide.MUDr. Jan Pláteník, Ph.D. 
5Tuesday13:30 - 15:00seminarMetabolism of saccharides.MUDr. Jan Pláteník, Ph.D. 
Friday14:15 - 16:30practical classesSaccharides II: Acidic hydrolysis of starch. Substrate specificity of amylase and sucrase. Polarimetry of glucose and fructose.MUDr. Jan Pláteník, Ph.D. 
6Tuesday13:30 - 15:00seminarOverview of basic methods used in biochemical lab: photometry, chromatography, electrophoresis, blotting. MUDr. Jan Pláteník, Ph.D. 
Friday14:15 - 16:30nothingFriday 29.3. Easter Holiday  
7Tuesday13:30 - 15:00seminarLipids: Biosynthesis and degradation of fatty acids doc. MUDr. Evžen Křepela, CSc. 
Friday14:15 - 16:30practical classesIntroduction to spectrophotometry: Absorption spectra of colored solutions. Calibration curve, factor calculation. MUDr. Jan Pláteník, Ph.D. 
8Tuesday13:30 - 15:00seminarRelationships between metabolism of lipids and saccharides. Starvation MUDr. Jan Pláteník, Ph.D. 
Friday14:15 - 16:30practical classesSaccharides III: Estimation of glycemia. Glucose in urine. MUDr. Jan Pláteník, Ph.D. 
9Tuesday13:30 - 15:00seminarMetabolism of amino acids: Transamination, deamination, synthesis of urea. MUDr. Jan Pláteník, Ph.D. 
Friday14:15 - 16:30practical classesLipids: Estimation of total cholesterol and triacylglycerols. Demonstration of double bonds in fatty acids. Test for malondialdehyde. MUDr. Jan Pláteník, Ph.D. 
10Tuesday13:30 - 15:00seminarRevision test: Core metabolic pathways MUDr. Jan Pláteník, Ph.D. 
11Tuesday13:30 - 15:00seminarPorphyrins and bile pigments doc. MUDr. Evžen Křepela, CSc. 
Friday14:15 - 16:30practical classesLiver tests: Direct and indirect bilirubin. Estimation of aminotransferases. MUDr. Jan Pláteník, Ph.D. 
12Tuesday13:30 - 15:00seminarMetabolism of calcium, phosphate, and other elements and vitamins necessary for mineralisation of bone and teeth. doc. MUDr. Ivan Matouš-Malbohan, CSc. 
Friday14:15 - 16:30practical classesBone and teeth: Estimation of calcium and phosphate in serum and urine. Isoenzymes of alkaline phosphatase. Properties of dental hydroxyapatite. MUDr. Jan Pláteník, Ph.D. 
13Tuesday13:30 - 15:00seminarTuesday 14.5. Rector's DayMUDr. Jan Pláteník, Ph.D. 
Friday14:15 - 16:30practical classesSaliva: Demonstration of selected components MUDr. Jan Pláteník, Ph.D. 
14Tuesday13:30 - 15:00seminarTooth: Chemical composition and structure doc. MUDr. Ivan Matouš-Malbohan, CSc. 
Friday14:15 - 16:30practical classesPractical credit test, credit MUDr. Jan Pláteník, Ph.D. 
15Friday14:15 - 16:30practical classesCredit 
Schedule semestral
Educational week Date From - To Education type Theme Teacher Files Note Hodnocení
120.02.202412:30 - 13:15nothingAnatomical Dissections  
227.02.202412:30 - 13:15nothingAnatomical Dissections  
305.03.202412:30 - 13:15lectureGeneral features of metabolism. Bioenergetics.MUDr. Jan Pláteník, Ph.D. 
412.03.202412:30 - 13:15lectureThe citric acid cycle. MUDr. Jan Pláteník, Ph.D. 
519.03.202412:30 - 13:15lectureDigestion, absorption, storage and transport of saccharides. MUDr. Jan Pláteník, Ph.D. 
626.03.202412:30 - 13:15lectureRegulation of metabolism of saccharides. MUDr. Jan Pláteník, Ph.D. 
702.04.202412:30 - 13:15lectureBiomembranes. Composed lipids.RNDr. Marek Vecka, Ph.D. 
809.04.202412:30 - 13:15lectureLipoproteins: Classification, properties and metabolism.MUDr. Martin Vejražka, Ph.D. 
916.04.202412:30 - 13:15lectureSteroids: Synthesis and metabolism. Steroid hormones. Bile acids.RNDr. Marek Vecka, Ph.D. 
1023.04.202412:30 - 13:15nothing  
1130.04.202412:30 - 13:15lectureConversion of amino acids to biologically active derivatives. MUDr. Jan Krtil 
1207.05.202412:30 - 13:15lectureThe connective tissue, bone and cartilage. Proteoglycans, collagen and elastin – metabolism. doc. MUDr. Ivan Matouš-Malbohan, CSc. 
1314.05.202412:30 - 13:15lectureSaliva doc. MUDr. Ivan Matouš-Malbohan, CSc. 
 
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