Laboratory Technique (b) - MC240C24
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harmonogram-b.png | rozpis prováděných úloh během semestru | prof. RNDr. Jan Kotek, Ph.D. |
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LT_6.3_eng.pdf | Textbook in English | Mgr. Michaela Fridrichová, Ph.D. |
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LT_6.4_cz.pdf | Textbook in Czech | Mgr. Michaela Fridrichová, Ph.D. |
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Laboratory technique is a basic practicum which unifies and extends laboratory skills obtained during high-school studies. It prepares students for further laboratory works. After passing the practicum, students are able to perform basic laboratory works and can operate common laboratory instruments (pH-meter, UV-Vis spectrophotometer, vacuum rotary evaporator), they are familiar with safety of work in the laboratory and they know how to treat data and write laboratory diary and reports of their work. Practicum takes place once a week in a block of four teaching hours; students work in pairs according to time schedule in such a way, that they pass all 5 tasks during the semester. Tasks include measurements of properties of unknown samples, synthetic and purification operations, and simple analytical procedures.
Last update: Fridrichová Michaela, Mgr., Ph.D. (03.09.2025)
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Textbook Jan Kotek: Laboratory technique, version 6, accessible in electronic form, see link above. Last update: Fridrichová Michaela, Mgr., Ph.D. (04.07.2022)
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To get credits it is mandatory to successfully finish entry test (safety rules and basic laboratory calculations), personally complete all 5 laboratory tasks, write all protocols in acceptable form and pass the final test. Last update: Fridrichová Michaela, Mgr., Ph.D. (03.09.2025)
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List of tasks
Task 1 – Crystallization Task 2 – Isolation of glycine and identification of unknown amino acid Task 3 – Identification and concentration determination of unknown organic acid Task 4 – Rectification and work with gases Task 5 – Spectrophotometric quantitative analysis Last update: Fridrichová Michaela, Mgr., Ph.D. (03.09.2025)
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A graduate of the course possesses a comprehensive understanding of basic laboratory equipment and instruments and, through the application of proficient working techniques, is capable of obtaining valuable results. With experience in executing the tasks outlined in the curriculum, the graduate can accurately assess the time requirements for specific laboratory activities, judiciously select appropriate laboratory glassware and equipment, and make informed decisions regarding the precision of required operations, such as determining whether to utilize pre-scales or analytical balances for a particular measurement or whether to employ a graduated cylinder or a pipette for liquid volume measurement. The graduate acknowledges the importance of maintaining a clean and meticulous laboratory environment while adhering to established safety protocols throughout their work. Furthermore, the graduate diligently documents their activities, skillfully processes results utilizing computer software, prepares graphical representations and tables, and composes original reports that reflect the outcomes of their work.
Last update: Fridrichová Michaela, Mgr., Ph.D. (03.09.2025)
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