Advanced course in Mycology - MB120T120
|
|
|
||
|
The course will be organized in AY 2024/2025 in Czech Republic during two extended weekends (Fri-Sun) in April. Localities for field trips are located around Tuchoměřice (Prague), following steps will be performed at the Faculty.
In this Academic year, the course is inspired by the great Bohemian mycologist ACJ Corda, who was not only a great naturalist, but also an excellent fungal taxonomist. We will follow his steps and during one field trip to "Tuchomeritz prope Pragam", we will collect natural substrate colonised by microfungi. Ascomycetes in both anamorphic and teleomorphic state, corticioid basidiomycetes and other fungi. During the second phase, we will identify these microfungi based on phenotype traits in field and in the lab using optical microscopes. We will also culture the material in damp chambers and try to isolate the fungi into pure cultures. During the course, molecular data (sequences of several molecular markers) will be obtained from fresh material and students will evaluate the results of identification from both phenotype and molecular data. Additional activities include work on small taxonomic projects that will be designed based on the material that we find. Students will work in teams of 2-3 students. Dates and approximate activities: Fri, 11th April - field trip to Tuchoměřice, collection of material Sat, 12th April - microscopy, identification, culturing, isolation into pure cultures, preparation of vouchers, when possible Sun, 13th April - extraction of DNA, PCR Fri, 18th April - evaluation of the isolation success, vouchers from damp chambers, editing of sequences Sat, 19th April - finishing projects, deposition of vouchers in PRC Sun, 20th April - presentation of results of projects Besides travelling costs, there will be no payment. Each of the students enrolled will be responsible for his/her own food and accommodation during the course. Last update: Koukol Ondřej, doc. Mgr., Ph.D. (21.01.2025)
|
|
||
|
Student participate on all field trips, collect their own material and identify it based on combination of phenotype and molecular data. Students also participate on preparation of fungarium vouchers and databasing. The last day of the course, students present results of their projects at a "conference". Last update: Koukol Ondřej, doc. Mgr., Ph.D. (21.01.2025)
|
|
||
|
It is highly recomended that students already successfully passed at least one basic course on Mycology (e.g. MB120P182) and have active knowledge of terminology used in mycology. Any previous experience with DNA analyses (simple similarity search, tree constructing) is also wellcome. This course is definitely NOT recomended as a first experience with fungi, field sampling and work with DNA sequences in taxonomy! Last update: Koukol Ondřej, doc. Mgr., Ph.D. (21.01.2025)
|