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Advanced methods in microscopy - CCOCA0090
Title:
Advanced methods in microscopy
Guaranteed by:
Department of Histology and Embryology 3FM CU (12-HIST)
Faculty:
Third Faculty of Medicine
Actual:
from 2020
Semester:
summer
Points:
1
E-Credits:
1
Examination process:
summer s.:
Hours per week, examination:
summer s.:0/15, C [HS]
Capacity:
unknown / unknown (10)
Min. number of students:
3
4EU+:
no
Virtual mobility / capacity:
no
Key competences:
State of the course:
not taught
Language:
English, Czech
Teaching methods:
full-time
Teaching methods:
full-time
Level:
Note:
course can be enrolled in outside the study plan enabled for web enrollment
Annotation -
--- Czech English
Last update: RNDr. Radek Pelc, D.Phil. (12.12.2019)
Special histological procedures. Special microscopy modalities. History of staining protocols (textile and cosmetic dyes versus staining of biological tissue in medicine).
Last update: MUDr. Petr Michenka (28.12.2019)
Speciální histologické postupy. Speciální mikroskopické modality. Historie barvících postupů (textilní a kosmetická barviva versus barvení biologické tkáně v medicíně).
Aim of the course -
--- Czech English
Last update: RNDr. Radek Pelc, D.Phil. (12.12.2019)
see OUTLINE ("ANOTACE")
Last update: RNDr. Radek Pelc, D.Phil. (12.12.2019)
viz ANOTACE
Course completion requirements -
--- Czech English
Last update: RNDr. Radek Pelc, D.Phil. (12.12.2019)
Being present at seminars
Last update: RNDr. Radek Pelc, D.Phil. (02.12.2019)
Prezence
Literature -
--- Czech English
Last update: RNDr. Radek Pelc, D.Phil. (12.12.2019)
IN-DEPTH BIOLOGICAL MICROSCOPY:
Inoué S. & Spring K. (1997) Video microscopy (2nd ed.). Springer, New York. ISBN: 978-0-306-45531-5 https://www.springer.com/gp/book/9780306455315
(may be borrowed from the teacher = Pelc R.)
SELECTION OF RECENTLY DEVELOPED APPROACHES IN HISTOLOGY AND MICROSCOPY:
Pelc R., Walz W. & Doucette J.R. (2020)
Neurohistology and imaging techniques.
Springer (Humana Press), New York
ISBN: 978-1-07-160426-7
https://www.springer.com/gp/book/9781071604267
(may be borrowed from the teacher = Pelc R.)
Last update: RNDr. Radek Pelc, D.Phil. (12.12.2019)
BIOLOGICKÁ MIKROSKOPIE PODROBNĚ:
Inoué S. & Spring K. (1997) Video microscopy (2nd ed.). Springer, New York. ISBN: 978-0-306-45531-5 https://www.springer.com/gp/book/9780306455315
(k zapůjčení u lektora = Pelc R.)
NĚKTERÉ NEJNOVĚJŠÍ POSTUPY V HISTOLOGII A MIKROSKOPII:
Pelc R., Walz W. & Doucette J.R. (2020)
Neurohistology and imaging techniques.
Springer (Humana Press), New York
ISBN: 978-1-07-160426-7
https://www.springer.com/gp/book/9781071604267
(k zapůjčení u lektora = Pelc R.)
Syllabus -
--- Czech English
Last update: RNDr. Radek Pelc, D.Phil. (12.12.2019)
- Immunohistochemistry
- EdU Click Kit (cell proliferation)
- Fluorescent labels (Fura-2, ...) a proteins (GFP, YFP, RFP, ...)
- Modulation contrast
- Ultramicroscopy (light-sheet microscopy)
- Fluorescence, confocal a two-photon microscopy
- Chemical (IR/Raman) microscopy (microspectroscopy)
- Digital photomicrography
Last update: RNDr. Radek Pelc, D.Phil. (12.12.2019)
- Imunohistochemie
- EdU Click Kit (sledování proliferace buněk)
- Fluorescenčních sondy (Fura-2, ...) a proteiny (GFP, YFP, RFP, ...)
- Modulační kontrast
- Ultramikroskopie (“světelný list”)
- Fluorescenční, konfokální a dvoufotonová mikroskopie
- Chemická (IČ/Ramanova) mikroskopie (mikrospektroskopie)
- Digitální mikrofotografie
Course completion requirements -
--- Czech English
Last update: RNDr. Radek Pelc, D.Phil. (12.12.2019)
Being present at seminars
Last update: RNDr. Radek Pelc, D.Phil. (02.12.2019)
Prezence