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Last update: prof. MUDr. Markéta Dušková, CSc. (16.06.2008)
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Last update: prof. MUDr. Markéta Dušková, CSc. (12.11.2008)
The objective of the project :
1. innervation of the tongue in patients with cleft and any statistically significant difference with the healthy population
2. qualitative and quantitative morphological differences of taste buds and nerve endings in the necropsy of embryos with cleft compared with the germs that do not have this defect
3. specification of the changes originating during embryogenesis and the others belonging to subsequent development |
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Last update: prof. MUDr. Markéta Dušková, CSc. (16.06.2008)
1. Bardach J. Salyer´s and Bardach´s atlas of Craniofacial and Cleft Surgery. Vol. II, Cleft Lip and Palate Surgery. Lippincott Williams & Wilkins, Philadelphia, USA 1999. 2. Dileo MD, Amedee RG: Disorders of taste and; smell. J La State Med Soc. 1994 Oct;146(10): 433-7. 3. Dušková M, Kozák J, Šmahel Z. et al. Augmentace obličejového skeletu u vrozených vývojových vad a u poúrazových nebo pooperačních deformit. Závěrečná zpráva výzkumného projektu grantu NK 4659-3 IGA MZČR, Praha, 2000. 4. Dušková M, Koťová M, Strnadel T. et al. Sekundární chirurgická a ortodontickoprotetická léčba u dospělých nemocných s rozštěpem rtu a patra. Závěrečná zpráva výzkumného projektu grantu NK 6653-3 IGA MZČR, Praha, 2003. 5. Dušková M, Urban F, Koťová M. et al. Rekonstrukce alveolárního defektu maxily u nemocných s rozštěpem v časné dospělosti. Závěrečná zpráva výzkumného projektu IGA MZČR NK 7186-3, Praha, 2004. 6. Dušková M, Laštovka M, Škodová E. et al. Korelace mezi výsledkem chirurgické léčby a tvorbou řeči u dospívajících nemocných s rozštěpem. Závěrečná zpráva výzkumného projektu IGA MZČR NR 8089-3, Praha, 2006. 7. Enlow DH, Hans MG: Essentials of Facial Growth. Edition I. W.B.Saunders Comp. Philadelphia, p.1-36, 57-99,146-166, 1996. 8. Koťová M. Vývoj a růst orofaciální soustavy. Dizert. kandidátská práce. Praha, 2002. 9. Letra A, Menezes R, Granjeiro JM, Vieira AR: Defining subphenotypes for oral clefts based on dental development. J Dent Res. 2007 Oct;86(10):986-91. 10. Mathes SJ. Plastic Surgery 2nd edition. Vol. IV. Pediatric Plastic Surgery. Saunders-Elsevier, Philadelphia, 2006. ISBN 0-7216-8815-2/978-0-7216-8815-2. 11. Nopoulos P et al.: Abnormal brain structure in children with isolated clefts of the lip or palate. Arch Pediatr Adolesc Med. 2007 Aug;161(8):753-8 12. Peterka M. Příčiny vzniku vrozených vad, jejich léčba a prevence, Praha, 2005. 13. Tolarová M. Cleft lip and palate, eMedicine, May 15, 2006. 14. Vokurková, J.: Rozštěpové vady obličeje, dizertační práce, LF MU Brno, 2000. |
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Last update: prof. MUDr. Markéta Dušková, CSc. (12.11.2008)
1. acknowledgement with the principles of scientific work 2. leadership in the search of published information, processing of the project and its results 3. processing of report 4. publication in the form of dissertation and possibly scientific article |
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Last update: prof. MUDr. Markéta Dušková, CSc. (12.11.2008)
1. at the least once monthly make partial output 2. after the completion of the project to provide results within 2 months 3. the final report within 4 months after the completion of the project, concept of publication in 8 months, the thesis within 12 months after completion of the project |
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Last update: prof. MUDr. Markéta Dušková, CSc. (12.11.2008)
Cleft lip and palate is one of the most common congenital disorders. Full rehabilitation of these patients requires multidisciplinary treatment, which begins immediately after birth and lasts until adulthood because of the congenital defect linked hypoplasia of affected area. On a comprehensive treatment gradually, depending on the postnatal development, health and growth affected, a specialist team is involved. It consists of the plastic surgeon, dentist (orthodontist, maxillofacial surgeon, and stomatological prosthetian), speech specialist, foniatrist, audiologist, and ultimately clinical psychologist . Cleft of primary and secondary palate lie in the failure of the various embryonic processes, which may occur together. To differentiate facial participating in all three germ strips. In patients with cleft follow-up to the birth defect hypoplasia of the affected tissue centrofaciální area. Based on the physiological growth is possible, to a certain extent, to predict the resulting secondary deformity. Embryological development of the face begins at the beginning of the fourth week around a large stomodea and lasts until the end of the eighth week of gestation. Process is extremely complex. During the short period of 4 weeks is an enormous demand on the coordination of cell separation, migration and interaction. The correct amount of tissue must be the right place at the right time - any error leads to disastrous consequences. At the end of embryonic period, the human form of the face, during the fetal period, the child develops the proportions of the face. While some clefts are found in atypical locations, the majority, as well as rare cleft, will occur at site of predictable embryonic lines. So in the normal lines of fusion of different tissue . Primarily the morphological character defect has severe negative consequences for the functioning swallowing, breathing, biting and the formation of speech. Due to the presence of a number of deformities in the orofacial area, it can be assumed the dysfunction of appetite, which is very stressful for the patient and may have a negative impact not only on its maintenance, but also the overall quality of life. Clinical project will be verified objectification morphological differences (the number and quality of nerve endings, mucous membrane structure and its papillae) and through examination of necropsy. The mucosa root tongue of dead embryos with the cleft is compared with the necropsy embryos that do not have this defect. Tissue obtained from the root of the language they are ready cuts, which after processing stained. Tests will be done standart histological and imunohistologickými methods with a focus on card acetylcholinesterasy, neuron-specific enolasy, synaptophysinu and Chromogranin. The planned number of samples in each group is at least thirty. The aim is therefore to the establishment of qualitative and quantitative changes in the receptors, their number, histology, histochemistry and immunohistochemical examination due to possible pathological changes. Tests will be conducted in both groups as well and the results will be compared and then statistically processed. |
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Last update: prof. MUDr. Markéta Dušková, CSc. (12.11.2008)
Study results, interest in the speciality, a personal interview |