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Last update: prof. RNDr. Petr Heřman, CSc. (19.02.2018)
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Last update: HERMAN/MFF.CUNI.CZ (07.05.2008)
Students will become familiar with advanced methods of emission spectroscopy and their applications in biomedical sciences. |
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Last update: prof. RNDr. Petr Heřman, CSc. (30.10.2019)
Oral exam. |
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Last update: T_FUUK (28.04.2008)
J.R.Lakowicz: Principles of Fluorescence Spectroscopy, 2nd Edition, Plenum Publ.Corp., New York, 1999
Vo-Dingh, CRC Biomedical Photonics Handbook, CRC Press, New York, 2003
M.G.Ormerod (ed.): Flow Cytomety - A Practical Approach, IRL Press, Oxford Univ.Press, 1890
J.R.Lakowicz: Topics in Fluorescence Spectroscopy Vol.3 - Biochemical Applications Plenum Publ.Corp., London 1992
J.R.Lakowicz: Topics in Fluorescence Spectroscopy Vol.4 - Probe Design and Chemical Sensing, Plenum Publ.Corp., London 1994
D.L.Taylor et al.(ed.): Applications of Fluorescence in the Biomedical Sciences Part I, II Alan R.Liss, Inc., New York 1986
V.T.Mason (ed.): Fluorescent and Luminescent Probes for Biological Activity - A Practical Guide to Technology for Quantitative Real-Time Analysis Academic Press, London 1993
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Last update: T_FUUK (12.05.2008)
lecture |
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Last update: prof. RNDr. Petr Heřman, CSc. (30.10.2019)
See syllabus. |
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Last update: prof. RNDr. Petr Heřman, CSc. (19.02.2018)
1. Fluorescence prnciples
2. Classical and advanced fluorescence techniques, steady state and time resolved emission spectroscopy, instrumentation for stedy state, time domain, and frequency domain fluorescence spectroscopy, laser photolysis (FRAP), fluorescence correlation spectroscopy (FCS), microfluorimetry, fluorescence lifetime imaging (FLIM), spectral imaging, multiphoton fluorescence and imaging, nonlinear optical methods for resolution enhancement, optical sectioning, 3D reconstruction of emitting objects.
3. Applications of the emission spectroscopy in biology, chemistry, medicine, and industry, imunofluorescence, etc.
4. Fluorescence of biopolymers.
5. Fluorescence sensors (principles and functin - FRET, quenching, polarization, spectral changes, etc.), enhaced fluorescence near metal nanostructures. |