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Course, academic year 2023/2024
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Emission Spectroscopy in Biophysics - NOOE004
Title: Emisní spektroskopie v biofyzice
Guaranteed by: Institute of Physics of Charles University (32-FUUK)
Faculty: Faculty of Mathematics and Physics
Actual: from 2019
Semester: summer
E-Credits: 3
Hours per week, examination: summer s.:2/0, Ex [HT]
Capacity: unlimited
Min. number of students: 4
4EU+: no
Virtual mobility / capacity: no
State of the course: not taught
Language: Czech
Teaching methods: full-time
Teaching methods: full-time
Note: course can be enrolled in outside the study plan
enabled for web enrollment
Guarantor: prof. RNDr. Petr Heřman, CSc.
Classification: Physics > Physics, Astronomy and Astrophysics, Biophysics and Chemical Physics, Ecology and Environmentalism, External Subjects, General Subjects, Geophysics, Mathematics for Physicists, Meteorology and Climatology, Mathematical and Computing Modelling in Physics, Nuclear and Subnuclear Physics, Optics and Optoelectronics, Solid State Physics, Surface Physics and P. of Ion.M., Teaching, Theoretical and Math. Physics, Biophysics and Chemical Physics
Incompatibility : NBCM113
Annotation -
Methods of emission spectroscopy and their applications in biomedical sciences. Fluorescence probes and markers, fluorescence sensors.
Last update: Heřman Petr, prof. RNDr., CSc. (19.02.2018)
Aim of the course -

Students will become familiar with advanced methods of emission spectroscopy and their applications in biomedical sciences.

Last update: HERMAN/MFF.CUNI.CZ (07.05.2008)
Course completion requirements -

Oral exam.

Last update: Heřman Petr, prof. RNDr., CSc. (30.10.2019)
Literature -

J.R.Lakowicz: Principles of Fluorescence Spectroscopy, 2nd Edition, Plenum Publ.Corp., New York, 1999

Vo-Dingh, CRC Biomedical Photonics Handbook, CRC Press, New York, 2003

M.G.Ormerod (ed.): Flow Cytomety - A Practical Approach, IRL Press, Oxford Univ.Press, 1890

J.R.Lakowicz: Topics in Fluorescence Spectroscopy Vol.3 - Biochemical Applications Plenum Publ.Corp., London 1992

J.R.Lakowicz: Topics in Fluorescence Spectroscopy Vol.4 - Probe Design and Chemical Sensing, Plenum Publ.Corp., London 1994

D.L.Taylor et al.(ed.): Applications of Fluorescence in the Biomedical Sciences Part I, II Alan R.Liss, Inc., New York 1986

V.T.Mason (ed.): Fluorescent and Luminescent Probes for Biological Activity - A Practical Guide to Technology for Quantitative Real-Time Analysis Academic Press, London 1993

Last update: T_FUUK (28.04.2008)
Teaching methods -


Last update: T_FUUK (12.05.2008)
Requirements to the exam -

See syllabus.

Last update: Heřman Petr, prof. RNDr., CSc. (30.10.2019)
Syllabus -

1. Fluorescence prnciples

2. Classical and advanced fluorescence techniques, steady state and time resolved emission spectroscopy, instrumentation for stedy state, time domain, and frequency domain fluorescence spectroscopy,

laser photolysis (FRAP), fluorescence correlation spectroscopy (FCS), microfluorimetry, fluorescence lifetime imaging (FLIM), spectral imaging, multiphoton fluorescence and imaging, nonlinear optical methods for resolution enhancement, optical sectioning, 3D reconstruction of emitting objects.

3. Applications of the emission spectroscopy in biology, chemistry, medicine, and industry, imunofluorescence, etc.

4. Fluorescence of biopolymers.

5. Fluorescence sensors (principles and functin - FRET, quenching, polarization, spectral changes, etc.), enhaced fluorescence near metal nanostructures.

Last update: Heřman Petr, prof. RNDr., CSc. (19.02.2018)
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