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Course, academic year 2023/2024
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Practical Course in Fluorescence Spectroscopy: Biophysical Applications - NBCM314
Title: Praktický kurs fluorescenční spektroskopie: biofyzikální aplikace
Guaranteed by: Institute of Physics of Charles University (32-FUUK)
Faculty: Faculty of Mathematics and Physics
Actual: from 2020
Semester: winter
E-Credits: 3
Hours per week, examination: winter s.:0/2, MC [HT]
Capacity: unlimited
Min. number of students: 2
4EU+: no
Virtual mobility / capacity: no
State of the course: not taught
Language: Czech
Teaching methods: full-time
Teaching methods: full-time
Note: course can be enrolled in outside the study plan
enabled for web enrollment
Guarantor: prof. RNDr. Petr Heřman, CSc.
Classification: Chemistry > Biochemistry, Physical Chemistry
Physics > Physics, Astronomy and Astrophysics, Biophysics and Chemical Physics, Ecology and Environmentalism, External Subjects, General Subjects, Geophysics, Mathematics for Physicists, Meteorology and Climatology, Mathematical and Computing Modelling in Physics, Nuclear and Subnuclear Physics, Optics and Optoelectronics, Solid State Physics, Surface Physics and P. of Ion.M., Teaching, Theoretical and Math. Physics, Biophysics and Chemical Physics
Annotation -
Practical training of basic and advanced experimental methods of fluorescence spectroscopy including data analysis. Time resolved methods in both time and frequency domains, FLIM FCCS, FAIM, multiphoton microscopy, etc. Students choose 4 problems from the offered list. The course is recommended for master and doctoral students.
Last update: Heřman Petr, prof. RNDr., CSc. (19.02.2018)
Aim of the course -

Students will learn practical applications of advanced fluorescence methods.

Last update: T_FUUK (07.05.2008)
Course completion requirements -

Active presence.

Last update: Heřman Petr, prof. RNDr., CSc. (30.10.2019)
Literature -

Večeř J., Heřman P. Kurs fluorescenční spektroskopie, learning texts, MFF UK, Praha 2006.

Last update: T_FUUK (28.04.2008)
Teaching methods -

One week practical training

Last update: T_FUUK (12.05.2008)
Requirements to the exam -

Completion of 4 experiments from the list.

Last update: Heřman Petr, prof. RNDr., CSc. (30.10.2019)
Syllabus -
List of optional experiments:
1) Measurements of fluorescence lifetimes in the frequency domain

2) Measurements of fluorescence lifetimes and fluorescence anisotropies in the time domain


4) Spectral FLIM


6) Two photon FLIM/FAIM

7) Basics of FCS

8) FLuorescence quenching

9) Parametric data analysis in the time domain

10) Parametric data analysis in the frequency domain

11) Data analysis by MEM

Last update: Heřman Petr, prof. RNDr., CSc. (30.10.2019)
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