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Methods of optical absorption spectroscopy, light scattering and Raman spectroscopy,
chiroptical and luminescence methods as applied in biophysical research.
Last update: G_F (07.01.2003)
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To learn students theoretical and technical basics of molecular optical spectroscopy and its application in study of biological targets. Last update: STEPJOS/MFF.CUNI.CZ (15.05.2008)
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Oral exam. Last update: Štěpánek Josef, prof. RNDr., CSc. (30.10.2019)
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O.Jelínek,P.Pančoška,J.Štěpánek: Metody optické spektroskopie (v uč. \"Experimentální metody biofyziky\",V.Prosser a kol.), Academia, Praha 1989
Parker C.A.: Photoluminescence of Solutions, Elsevier, Amsterdam (1986) - ruský překlad Fotoljuminescencija rastvorov, Izd. nauka, Moskva (1972)
Vacek K., Pantoflíček J., Jelínek O.: Luminiscence I a II, SPN Praha (1972)
Jelínek O.: Experimentální metody biofyziky III - Luminiscence, SPN Praha (1983)
Lakowicz J.R.: Principles of Fluorescence Spectroscopy, Plenum Press, New York, second edition (1999)
Alfano E.R. (ed.): Biological Events Probed by Ultrafast Laser, Spectroscopy, Acad. Press, New York- London (1982)
Žaloudek F., Plášek J., Sladký P.: Vybrané metody optické spektroskopie, SPN Praha (1982)
Nepraš M., Titz M.: Základy teorie elektronových spekter , SNTL Praha (1983). Last update: STEPJOS/MFF.CUNI.CZ (15.05.2008)
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lecture Last update: STEPJOS/MFF.CUNI.CZ (15.05.2008)
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Zkouška je ústní. Požadavky ke zkoušce jsou v rozsahu zveřejněného sylabu. Last update: Štěpánek Josef, prof. RNDr., CSc. (16.10.2017)
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Conversion of the excitation energy to luminescence - general rules: Jabloňski diagram, radiative and nonradiative transitions, fluorescence and phosphorescence, internal conversion and intersystem crossing
Basic characteristics of fluorescence: intensity, excitation and emission spectra, quantum yield, fluorescence lifetime and its relation to absorption, polarized fluorescence in solution, fluorescence anisotropy, polarization and anisotropy terms, photoselection, Perrin equation
Measurements of the steady-state spectra: basic fluorimeter set-up, excitation light sources, filters and polarizers, monochromator, analog detection and photon counting, correction of emission spectra, quantum yield measurements
Fluorescence lifetime measurements: time domain: fluorescence intensity decay, response function, convolution integral, magic angle, average lifetime, time-correlated single-photon counting method, streak camera; frequency domain: basic principles, modulation and phase dependence for single lifetime decay, frequency domain fluorimeter set-up
Solvent effects on emission spectra: general effect, Lippert equation, specific interactions, temperature effects
Fluorescence quenching: dynamic quenching, collisional quenching, Stern-Volmer equation, static quenching, applications of quenching to proteins
Fluorescence energy transfer: transfer rate from donor to acceptor, Főrster distance, overlap integral, orientation factor, molecular distance measurements
Excimer fluorescence: excimer, exciplex, two-state model, basic spectral and lifetime characteristics
Fluorophores: intrinsic or natural, extrinsic, protein fluorescence, fluorescent probes
Last update: T_FUUK (11.05.2004)
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