Subjects

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Preparation of Biological Samples - NAFY080

Title:	Příprava biologických vzorků
Guaranteed by:	Department of Chemical Physics and Optics (32-KCHFO)
Faculty:	Faculty of Mathematics and Physics
Actual:	from 2020
Semester:	summer
E-Credits:	3
Hours per week, examination:	summer s.:2/0, Ex [HT]
Capacity:	unlimited
Min. number of students:	unlimited
4EU+:	no
Virtual mobility / capacity:	no
State of the course:	taught
Language:	Czech
Teaching methods:	full-time
Teaching methods:	full-time
Note:	enabled for web enrollment

Guarantor:	doc. RNDr. Roman Dědic, Ph.D. doc. RNDr. Jakub Pšenčík, Ph.D.

Opinion survey results Examination dates SS schedule Noticeboard

Annotation -

Last update: RNDr. Vojtěch Kapsa, CSc. (14.05.2020)

The course provides introduction to the principles and use of basic chemical and technological procedures used in the preparation and storage of biological samples. The course is in the form of lectures and practical demonstrations. Themes: Importance of separation methods, classification and selection. Extraction, precipitation, centrifugation, dialysis, filtration, reverse osmosis, chromatography (types), electrophoresis, crystallization, distillation, lyophilization. pH measurement, oxygen concentration, preparation of liposomes. Cell cultures, tissue models.

Syllabus -

Last update: doc. RNDr. Roman Dědic, Ph.D. (14.05.2020)

1. Safety when working with biological material

2. Separation methods

mechanical disruption

extraction

precipitation

sedimentation and centrifugation

filtration, dialysis, molecular sieves

distillation

lyophilization

crystallization

chromatography (gas, liquid, HPLC, IEC, gel, affinity, thin layer)

electrophoresis

3. Cell cultures

4. Tissue models, CAM

5. Rules for working with laboratory animals