Introduction to mass spectrometry - MC270P71
Title: Úvod do hmotnostní spektrometrie
Czech title: Úvod do hmotnostní spektrometrie
Guaranteed by: Department of Organic Chemistry (31-270)
Faculty: Faculty of Science
Actual: from 2020 to 2023
Semester: winter
E-Credits: 3
Examination process: winter s.:
Hours per week, examination: winter s.:2/0, Ex [HT]
Capacity: unlimited
Min. number of students: 3
4EU+: no
Virtual mobility / capacity: no
State of the course: taught
Language: Czech
Explanation: Pro distanční výuku bude používána platforma MS Teams a Moodle.
Note: enabled for web enrollment
Guarantor: RNDr. Martin Štícha, Ph.D.
Teacher(s): RNDr. Martin Štícha, Ph.D.
Opinion survey results   Examination dates   WS schedule    E-learning course
Annotation -
Special course for pre-graduate and graduate students. In an introductory part the students are familiarized with fundamental construction schemes of mass-spectrometer. The survey of mass spectrometric instrumentation used at present is given. The following part is aimed at the interpretation of electron impact mass-spectra of organic compounds. The fundamentals of ion fragmentation are discussed. Theoretical considerations are accompanied with the examples of real mass-spectra and the explanation of important fragmentation pathways.
Last update: Štícha Martin, RNDr., Ph.D. (27.05.2024)
Literature -

Interpretation of Mass Spectra, F. W. McLafferty, University Science Books, Mill Valley, CA %94941, 1980.

Mass spectrometry for chemists and biochemists, Johnstone Robert A.

Mass spectrometry, Gross J. H.

Last update: Štícha Martin, RNDr., Ph.D. (27.10.2019)
Requirements to the exam -
The study is completed with an exam in the form of a written test of 60 minutes. The test consists of 4 questions. Each question is rated 5 points. The minimum number of points required to pass the exam is 12 (60%).
Last update: Štícha Martin, RNDr., Ph.D. (27.10.2019)
Syllabus -

Introduction. Experimental design of mass spectrometer. Survey of ionization techniques. Electron impact ionization. Chemical ionization. Other ionization techniques. Mass analyzers. Magnetic mass analyzer. Electric analyzer. Quadrupole analyzer. Ion trap. Time of flight analyzer. Detectors. Multipliers. Scintilation photomultipliers.

Mass spectrum. Molecular ion. Analysis of isotopic composition. Stable izotopes. Determination of number of rings plus double bonds from elemetal formula.

Theory of fragmentation. Charge and unpaired electron localization in molecular ion. Molecular ion fragmentation, fragmentation paths. Mechanisms of fragmetation of organic molecules. Sigma bond dissociation. Alfa cleavage initiated by radical-site. Cleavage initiated by charge-site. Decompositions of cyclic structures. Rearrangements. Radical-site rearrangements. Charge-site rearrangements.

Last update: KROUTIL (19.04.2006)