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The course gives an introduction to the principles of fluorescence and highlights relevant applications mainly in biology. The course is held every week, always two hours. The lecturer prefers to teach that subject in English. However, for the case the students wish that the course is held in Czech language, it will be held in Czech language.
Last update: Zusková Iva, doc. RNDr., CSc. (04.05.2012)
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Hof Martin, Hutterer R., Fidler V: Fluorescence Spectroscopy in Biology. pp. 290, Springer Verlag, Heidelberg, 2004 (ISBN 3-540-22338-x).
Lakowicz, Joseph R.: Principles of Fluorescence Spectroscopy. pp1255, Springer Verlag, Heidelberg,2006 (ISBN 978-0-387-31278-1)
Moreover, all lectures are available on the internet: http://www.jh-inst.cas.cz/~fluorescence/lectures.html Last update: Zusková Iva, doc. RNDr., CSc. (04.05.2012)
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The examination for that course is done orally in form of an individual conversation with the lecturer. Last update: Zusková Iva, doc. RNDr., CSc. (04.05.2012)
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1. Fluorescence spectroscopy and microscopy for biology and medicine 2. Absorption, Excitation and Emission Spectra, Quantum Yield 3. Fluorescence Lifetimes 4. Quenching of Fluorescence 5. Fluorescence Polarization 6. Effects of fluorophore's environment on its spectra 7. Förster Energy Transfer, Excimer Fluorescence, Fluorescent Proteins 8. Fluorescence microscopy I, Basic concepts of optical microscopy 9. Fluorescence microscopy II, Advanced approaches 10. Fluorescence microscopy III, Fluorescence correlation spectroscopy (FCS) 11. Photodynamic Therapy
Moreover, all lectures are available on the internet: http://www.jh-inst.cas.cz/~fluorescence/lectures.html Last update: Zusková Iva, doc. RNDr., CSc. (04.05.2012)
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