SubjectsSubjects(version: 953)
Course, academic year 2023/2024
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Fluoroscence spectroscopy in biological research - MC260P85
Title: Fluorescenční spektroskopie v biologickém výzkumu
Czech title: Fluorescenční spektroskopie v biologickém výzkumu
Guaranteed by: Department of Physical and Macromolecular Chemistry (31-260)
Faculty: Faculty of Science
Actual: from 2015
Semester: winter
E-Credits: 3
Examination process: winter s.:
Hours per week, examination: winter s.:2/0, Ex [HT]
Capacity: unlimited
Min. number of students: 3
4EU+: no
Virtual mobility / capacity: no
State of the course: taught
Language: Czech
Note: enabled for web enrollment
Guarantor: prof. Martin Hof, Ph.D., DSc.
Teacher(s): prof. Martin Hof, Ph.D., DSc.
Annotation -
The course gives an introduction to the principles of fluorescence and highlights relevant applications mainly in biology. The course is held every week, always two hours. The lecturer prefers to teach that subject in English. However, for the case the students wish that the course is held in Czech language, it will be held in Czech language.
Last update: Zusková Iva, doc. RNDr., CSc. (04.05.2012)
Literature -

Hof Martin, Hutterer R., Fidler V: Fluorescence Spectroscopy in Biology. pp. 290, Springer Verlag, Heidelberg, 2004 (ISBN 3-540-22338-x).

Lakowicz, Joseph R.: Principles of Fluorescence Spectroscopy. pp1255, Springer Verlag, Heidelberg,2006 (ISBN 978-0-387-31278-1)

Moreover, all lectures are available on the internet: http://www.jh-inst.cas.cz/~fluorescence/lectures.html

Last update: Zusková Iva, doc. RNDr., CSc. (04.05.2012)
Requirements to the exam -

The examination for that course is done orally in form of an individual conversation with the lecturer.

Last update: Zusková Iva, doc. RNDr., CSc. (04.05.2012)
Syllabus -

1. Fluorescence spectroscopy and microscopy for biology and medicine

2. Absorption, Excitation and Emission Spectra, Quantum Yield

3. Fluorescence Lifetimes

4. Quenching of Fluorescence

5. Fluorescence Polarization

6. Effects of fluorophore's environment on its spectra

7. Förster Energy Transfer, Excimer Fluorescence, Fluorescent Proteins

8. Fluorescence microscopy I, Basic concepts of optical microscopy

9. Fluorescence microscopy II, Advanced approaches

10. Fluorescence microscopy III, Fluorescence correlation spectroscopy (FCS)

11. Photodynamic Therapy

Moreover, all lectures are available on the internet: http://www.jh-inst.cas.cz/~fluorescence/lectures.html

Last update: Zusková Iva, doc. RNDr., CSc. (04.05.2012)
 
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