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Last update: doc. RNDr. Iva Zusková, CSc. (23.05.2019)
and has become one of the indispensable methods in biophysics. The aim of this course is to make students familiar with this field. Emphasis is placed on understanding the physico-chemical principles on which these methods are based. The usefulness of these fluorescence techniques is demonstrated on many practical examples from the field of biophysics. |
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Last update: doc. RNDr. Radek Šachl, Ph.D. (24.05.2019)
1. J. R. Lakowicz, Principles of fluorescence spectroscopy, Springer, 3rd edn., 2006. 2. B. Valeur, Molecular Fluorescence Principles and Applications, Wiley-VCH Verlag GmbH, New York, 2001. |
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Last update: doc. RNDr. Iva Zusková, CSc. (23.05.2019)
Oral exam covering the sylabus |
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Last update: doc. RNDr. Iva Zusková, CSc. (23.05.2019)
1) Introduction into fluorescence: Jablonski diagram; absorption and emission spectra; fluorescent 3) Single particle tracking (SPT): diffusion in two dimensions; random walk; MSD diagrams; different |