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Clinical biochemistry - practical course - MC250C44
Title: Praktikum z klinické biochemie
Czech title: Praktikum z klinické biochemie
Guaranteed by: Department of Biochemistry (31-250)
Faculty: Faculty of Science
Actual: from 2023
Semester: winter
E-Credits: 6
Examination process: winter s.:
Hours per week, examination: winter s.:0/4, C [HT]
Capacity: 24
Min. number of students: unlimited
4EU+: no
Virtual mobility / capacity: no
State of the course: taught
Language: Czech, English
Explanation: Požadavek: očkování proti hepatitidě B+ Splněná zkouška z Klinické a analytické biochemie (prerekvizita)
Note: enabled for web enrollment
Guarantor: prof. RNDr. Markéta Martínková, Ph.D.
Teacher(s): prof. RNDr. Petr Hodek, CSc.
prof. RNDr. Markéta Martínková, Ph.D.
Mgr. Artur Sergunin
Class: Rekonstrukce místnosti č.203, Hlavova 8
Pre-requisite : MC250P19B
Annotation -
The practicum is intended for students of primarily chemical study programs with an interest in clinical biochemistry, as an extension and practice of knowledge acquired in a lecture on Clinical and Analytical Biochemistry. The aim of the practical course is to introduce future workers in clinical laboratories to fundamental methods of determination of compounds in blood serum and urine.
Last update: Martínková Markéta, prof. RNDr., Ph.D. (09.09.2021)
Literature - Czech

Reference: Klinická biochemie. Edited by Jaroslav Racek. 2., přeprac. vyd. Praha : Galén, 2006. 329 s. ISBN 80-7262-324-9,
Textbook of biochemistry with clinical correlations. Edited by Thomas M. Devlin. 6th ed. ISBN 978-0-471-67808-3
František Novák - Úvod do klinické biochemie 2002, Universita Karlova v Praze, Nakladatelství Karolinum ISBN 80-246-0366-7
Tietz Textbook of Clinical Chemistry, eds.: C.A. Burtis, E.R. Ashwood, W.B. Saunders Company, 1994, Philadelphia, London, ISBN 0 7216 4472 4

 

Podklady k praktiku jsou deponovány na Moodle: http://dl2.cuni.cz/course/view.php?id=1679

Last update: Hodek Petr, prof. RNDr., CSc. (09.09.2014)
Requirements to the exam -

Requirements for granting the credits (it is necessary to meet all three requirements)
1. Active participation in practice course, carefully conducted laboratory diary (work report), results of the individual tasks - in total more than 90% (to be assessed at the end of each lab day)

2. Preparation of a detailed protocol from one selected task - more than 80% (at the end of the course)

3. Final test - more than 80% (at the end of the course)

Last update: Martínková Markéta, prof. RNDr., Ph.D. (09.09.2021)
Syllabus -

Introduction to safety program and identification of hazards in clinical laboratory - first lecture for both groups together will be held 4. 10. 2024 - information about practical course organization, introduction and so on.

Later during the practical course you will performe (all students will join a single group - no parallels and the dates are following:

1. analysis of glucose in serum - 18.10.2024

2. analysis of proteins in serum - 18.10.2024

3. determination of cholesterol - 1.11.2024

4. determination of triacylglycerol - 1.11.2024

5. analysis of urea - 15.11.2024

6. analysis of creratinine - 15.11.2024

7. determibnation of enzyme activities in serum (LD) - 29.11.2024

8. analysis of bilirubine - 29.11.2024

9. analysis of direct bilirubine - 29.11.2024

10. immunochemical quantification of antibodies in serum or antigen in a sample by ELISA kit (prof. Hodek) - 13.12.2024

 

 

Last update: Martínková Markéta, prof. RNDr., Ph.D. (19.08.2024)
Entry requirements -

1. hepatitis B vaccination

2. fulfilled prerequisite - MC250P19B Clinical and Analytical Biochemistry

3. attendance at the first class - occupational safety training

Last update: Martínková Markéta, prof. RNDr., Ph.D. (01.10.2024)
Learning outcomes -
  • The student determines the patient's serum glucose concentration and discusses the accuracy, precision, and clinical correlations of the value obtained.
  • The student determines the amount of total protein in a patient's serum and discusses the accuracy, precision, and clinical correlations of the value obtained.
  • The student determines the amount of total cholesterol in a patient's serum and discusses the accuracy, precision, and clinical correlations obtained for the value obtained.
  • The student determines the serum triacylglycerol concentration of a patient and discusses the obtained accuracy, precision, and clinical correlations of the value obtained.
  • The student discusses the correlation of the determined values of the patient's serum triacylglycerol concentration and cholesterol concentration and discusses the clinical correlations and suggests additional methods of examining the patient.
  • The student determines the serum and urine creatinine concentration of a patient and discusses the accuracy, precision, and clinical correlations of the value obtained.
  • The student calculates the creatinine clearance of a patient along with the obtained serum urea concentration of the patient discusses clinical correlations and suggests other methods of examining the patient.
  • The student determines the serum urea concentration of a patient and discusses the accuracy, precision, and clinical correlations of the value obtained.
  • The student determines the lactate dehydrogenase activity in a patient's serum and discusses the accuracy, precision, and clinical correlations obtained.
  • The student determines the concentration of total bilirubin in the serum of a patient and discusses the accuracy, precision, and clinical correlations of the value obtained.
  • The student determines the concentration of direct and conjugated bilirubin in the serum of a patient and discusses the accuracy, precision, and clinical correlations obtained.
  • The student evaluates the correlation of the determined values of the concentration of total bilirubin, the concentration of its individual fractions, and the obtained values of lactate dehydrogenase activity in the patient's serum, discusses the clinical correlations, and suggests additional methods of examining the patient.
  • The student performes immunochemical determination of antibodies in serum or antigens in samples using an ELISA kit.
Last update: Martínková Markéta, prof. RNDr., Ph.D. (01.10.2024)
 
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