Clinical biochemistry - practical course - MC250C44
Title: Praktikum z klinické biochemie
Czech title: Praktikum z klinické biochemie
Form of teaching: practical classes
Guaranteed by: Department of Biochemistry (31-250)
Faculty: Faculty of Science
Actual: from 2025
Duration in semesters: 1
Semester: winter
E-Credits: 6
Examination process: winter s.:
Hours per week, examination: winter s.:0/4, C [HT]
Capacity: 34
Maximum number of enrolled students: 34
Min. number of students: unlimited
4EU+: no
Virtual mobility / capacity: no
State of the course: taught
Language: Czech, English
Explanation: Požadavek: očkování proti hepatitidě B+ Splněná zkouška z Klinické a analytické biochemie (prerekvizita)
Repeated enrollment: 2 / 2 / 2 / 2
Note: enabled for web enrollment
Guarantor: prof. RNDr. Markéta Martínková, Ph.D.
Teacher(s): prof. RNDr. Petr Hodek, CSc.
prof. RNDr. Markéta Martínková, Ph.D.
Mgr. Dominik Pašek
Mgr. Artur Sergunin
Class: Rekonstrukce místnosti č.203, Hlavova 8
Pre-requisite : MC250P19B
Opinion survey results   WS schedule    E-learning course
Annotation -
The practicum is intended for students of primarily chemical study programs with an interest in clinical biochemistry, as an extension and practice of knowledge acquired in a lecture on Clinical and Analytical Biochemistry. The aim of the practical course is to introduce future workers in clinical laboratories to fundamental methods of determination of compounds in blood serum and urine.
Last update: Martínková Markéta, prof. RNDr., Ph.D. (09.09.2021)
Literature - Czech

Reference: Klinická biochemie. Edited by Jaroslav Racek. 2., přeprac. vyd. Praha : Galén, 2006. 329 s. ISBN 80-7262-324-9,
Textbook of biochemistry with clinical correlations. Edited by Thomas M. Devlin. 6th ed. ISBN 978-0-471-67808-3
František Novák - Úvod do klinické biochemie 2002, Universita Karlova v Praze, Nakladatelství Karolinum ISBN 80-246-0366-7
Tietz Textbook of Clinical Chemistry, eds.: C.A. Burtis, E.R. Ashwood, W.B. Saunders Company, 1994, Philadelphia, London, ISBN 0 7216 4472 4

 

Podklady k praktiku jsou deponovány na Moodle: http://dl2.cuni.cz/course/view.php?id=1679

Last update: Hodek Petr, prof. RNDr., CSc. (09.09.2014)
Course assessment methods and requirements for successful completion, grading scheme -

Requirements for granting the credits (it is necessary to meet all three requirements)
1. Active participation in practice course, carefully conducted laboratory diary (work report), results of the individual tasks - in total more than 90% (to be assessed at the end of each lab day)

2. Preparation of a detailed protocol from one selected task - more than 80% (at the end of the course)

3. Final test - more than 80% (at the end of the course)

Last update: Martínková Markéta, prof. RNDr., Ph.D. (09.09.2021)
Syllabus -

Introduction to safety regulations for working in a clinical laboratory, organizational guidelines, and verification of HBV vaccination certificates—Fri, Oct. 2, 2026, starting at 8:30 a.m.—please attend—attendance is mandatory! You will not be able to complete the course without attending this introductory session!


Subsequently, during the practical sessions, you will complete the following assignments (please arrange your personal schedule so that you can complete all assignments!!!)


1st parallel group
1.     Workplace Safety, introductory session Fri, Oct. 2, 2026, starting at 8:30 a.m. 
2.     Glucose determination – Oct. 9, 2026, at 8:00 a.m.
3.     Total protein determination – Oct. 9, 2026, at 8:00 a.m.
4.     Total cholesterol determination – Oct. 23, 2026, at 8:00 a.m.
5.     Triacylglycerol measurement - October 23, 2026, starting at 8:00 a.m.
6.     Creatinine measurement and calculation of creatinine clearance - November 6, 2026, starting at 8:00 a.m.
7.     Urea measurement - November 6, 2026, starting at 8:00 a.m.
8.     Lactate dehydrogenase activity measurement - November 20, 2026, starting at 8:00 a.m.
9.     Total bilirubin measurement - November 20, 2026, starting at 8:00 a.m.
10.   Direct (conjugated) bilirubin measurement - November 20, 2026, starting at 8:00 a.m.
11.   Immunochemical determination of antibodies in serum or antigens in samples using an ELISA kit (Prof. Hodek) – November 20 (theory starting at 1:30 p.m.) and December 4, 2026, starting at 1:00 p.m.

2nd parallel group
1.     Laboratory Safety, introductory session: Friday, October 2, 2026, starting at 8:30 a.m. 
2.     Glucose determination – October 9, 2026, starting at 9:00 a.m.
3.     Total protein determination – Oct. 9, 2026, starting at 9:00 a.m.
4.     Total cholesterol determination – Oct. 23, 2026, starting at 9:00 a.m.                                                                                                                                                                                         5.    Triacylglycerol Assay - October 23, 2026, starting at 9:00 a.m.
6.     Creatinine Assay and Calculation of Creatinine Clearance - November 6, 2026, starting at 9:00 a.m.
7.     Urea Assay - November 6, 2026, starting at 9:00 a.m.
8.     Lactate Dehydrogenase Activity Assay - November 20, 2026, starting at 9:00 a.m.
9.     Total Bilirubin Assay - November 20, 2026, starting at 9:00 a.m.
10.   Direct (conjugated) bilirubin measurement - November 20, 2026, starting at 9:00 a.m.
11.   Immunochemical determination of antibodies in serum or antigens in samples using an ELISA kit (Prof. Hodek) – November 20 (theory starting at 1:30 p.m.) and December 11, 2026, starting at 1:00 p.m.

 

Last update: Martínková Markéta, prof. RNDr., Ph.D. (16.09.2026)
Learning outcomes -
  • The student determines the patient's serum glucose concentration and discusses the accuracy, precision, and clinical correlations of the value obtained.
  • The student determines the amount of total protein in a patient's serum and discusses the accuracy, precision, and clinical correlations of the value obtained.
  • The student determines the amount of total cholesterol in a patient's serum and discusses the accuracy, precision, and clinical correlations obtained for the value obtained.
  • The student determines the serum triacylglycerol concentration of a patient and discusses the obtained accuracy, precision, and clinical correlations of the value obtained.
  • The student discusses the correlation of the determined values of the patient's serum triacylglycerol concentration and cholesterol concentration and discusses the clinical correlations and suggests additional methods of examining the patient.
  • The student determines the serum and urine creatinine concentration of a patient and discusses the accuracy, precision, and clinical correlations of the value obtained.
  • The student calculates the creatinine clearance of a patient along with the obtained serum urea concentration of the patient discusses clinical correlations and suggests other methods of examining the patient.
  • The student determines the serum urea concentration of a patient and discusses the accuracy, precision, and clinical correlations of the value obtained.
  • The student determines the lactate dehydrogenase activity in a patient's serum and discusses the accuracy, precision, and clinical correlations obtained.
  • The student determines the concentration of total bilirubin in the serum of a patient and discusses the accuracy, precision, and clinical correlations of the value obtained.
  • The student determines the concentration of direct and conjugated bilirubin in the serum of a patient and discusses the accuracy, precision, and clinical correlations obtained.
  • The student evaluates the correlation of the determined values of the concentration of total bilirubin, the concentration of its individual fractions, and the obtained values of lactate dehydrogenase activity in the patient's serum, discusses the clinical correlations, and suggests additional methods of examining the patient.
  • The student performes immunochemical determination of antibodies in serum or antigens in samples using an ELISA kit.
Last update: Martínková Markéta, prof. RNDr., Ph.D. (01.10.2024)
Course registration requirements -

1. hepatitis B vaccination

2. fulfilled prerequisite - MC250P19B Clinical and Analytical Biochemistry

3. attendance at the first class - occupational safety training

Last update: Martínková Markéta, prof. RNDr., Ph.D. (01.10.2024)
The course does not include work placement