|
|
|
||
Practical course in biochemistry is provided by Daniel Kavan, Veronika Hýsková, Josef Chmelík and Kateřina
Bělonožníková . The students are dealing with basic biochemical methods used for protein and nucleic acid isolation and their characterization (extraction, centrifugation, precipitation, dialysis, protein content determination). Gel filtration is used for protein purification from a mixture or for relative molecular mass determination. The students determine the relative molecular mass of proteins by SDS polyacrylamide gel electrophoresis. An important part of the practical course is dealing with enzymes, determination of Michaelis constant, maximal reaction rate, pH optimum, activation and inhibition, electrophoretic separation of isoenzymes and their detection in the gel. Last update: Kavan Daniel, RNDr., Ph.D. (31.01.2022)
|
|
||
Laboratory tasks manual provided as pdf file. Last update: Čermáková Michaela, RNDr., Ph.D. (19.04.2021)
|
|
||
Work on the laboratory task will be allowed only to students who demonstrate a sufficient understanding of the principles of tasks. It is necessary to consult obtained results with pedagogical staff before leaving the laboratory. The protocol must be submitted no later than the beginning of the next practice, otherwise the task will be forfeited. It is advisable to send the electronic version of the protocol by email at least 24 hours before the next laboratory practice (pdf format). In case of corrections, the protocol must be approved without mistakes within three weeks of the task being elaborated. At most one approved protocol may be missing at the time of the credit test.
To obtain the credit it is necessary to:
Last update: Čermáková Michaela, RNDr., Ph.D. (19.04.2021)
|
|
||
1. Isolation of a protein - enzyme, determination of specific activity 2. Gel chromatography of proteins (separation, relative molecular mass determination) 3. SDS - polyacrylamide electrophoresis (determination of relative molecular mass of proteins) 4. Practical enzymology - kinetic characteristics, Michaelis constants, maximal reaction rate, pH optimum, activation and inhibition of enzymes, electrophoretic separation of isoenzymes and detection of the enzyme activity in gel 5. DNA manipulation (restriction analysis) 6. Oral presentation of one experiment Last update: Čermáková Michaela, RNDr., Ph.D. (19.04.2021)
|