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Practical course in biochemistry - MC250C32

Title:	Biochemické praktikum pro učitele
Czech title:	Biochemické praktikum pro učitele
Guaranteed by:	Department of Biochemistry (31-250)
Faculty:	Faculty of Science
Actual:	from 2021
Semester:	winter
E-Credits:	4
Examination process:	winter s.:
Hours per week, examination:	winter s.:0/3, C [HT]
Capacity:	unlimited
Min. number of students:	unlimited
4EU+:	no
Virtual mobility / capacity:	no
State of the course:	cancelled
Language:	Czech
Note:	enabled for web enrollment

Guarantor:	RNDr. Daniel Kavan, Ph.D.
Is complex co-requisite for:	MC250C03, MC250C03E, MC250C17A, MC250C17E

Opinion survey results Examination dates Schedule

Annotation -

Practical course in biochemistry is provided by Helena Ryšlavá, Alice Šonská, Simona Eklová, Tomáš Kučera, Jiří Liberda

The students are dealing with basic biochemical methods. There are methods used for protein and nucleic acid isolation and their characterization (extraction, centrifugation, precipitation, dialysis). Ion exchange chromatography and gel filtration are used for protein purification from a complex mixture. The students have to determine the relative molecular mass of a protein by SDS polyacrylamide electrophoresis comparing the mobility of it with those of proteins with

Last update: SIMONAT (17.12.2002)

Literature - Czech

Laboratorní cvičení z biochemie, editoři: Doc. RNDr. M. Kodíček, CSc. a Doc. RNDr. O. Valentová, CSc.; Praha 2000

Základní praktikum z biochemie, J. Barthová, D. Sofrová, M. Tichá; Praha 1984

Last update: SIMONAT (17.12.2002)

Syllabus -

Isolation of a protein

Gel chromatography

Ion exchange chromatography

SDS - polyacrylamide electrophoresis

Practical enzymology - kinetic characteristics, apparent Michaelis constants, pH optimum, specific activities, detection of the enzyme activity in gels

Last update: SIMONAT (25.04.2002)