Practical Class in Biochemistry and Biology of Microorganisms - MC250C09E

• Title: Practical Class in Biochemistry and Biology of Microorganisms
• Czech title: Praktická cvičení z biochemie a biologie mikroorganismů
• Guaranteed by: Department of Biochemistry (31-250)
• Faculty: Faculty of Science
• Actual: from 2024
• Semester: summer
• E-Credits: 2
• Examination process: summer s.:
• Hours per week, examination: summer s.:0/3, C [DS]
• Capacity: 1
• Min. number of students: unlimited
• 4EU+: no
• Virtual mobility / capacity: no
• State of the course: taught
• Language: English
• Is provided by: MC250C09N
• Explanation: If interested in attending the course please contact T. Jecmen ([e-mail]). The number of participants may be increased.
• Note: enabled for web enrollment
• Guarantor: RNDr. Tomáš Ječmen, Ph.D.; Ing. Adriana Osičková, Ph.D.
• Teacher(s): RNDr. Věra Černá, Ph.D.; doc. RNDr. Olga Heidingsfeld, CSc.; RNDr. Tomáš Ječmen, Ph.D.; Ing. Adriana Osičková, Ph.D.; Mgr. Roman Tuzhilkin
• Class: Vysokokapacitní centrifuga s příslušenstvím; Stolní centrifuga s příslušenstvím; Původní předmět
• Pre-requisite : {At least one of the following subjects: MC250C01N, MC250C01, MC240C86, MC240C96}
• Co-requisite : MC250P08
• Incompatibility : MC250C09

Annotation

In 3-day practical course, students learn basic techniques of microorganism isolation, cultivation, manipulation, and preventing their contamination. Primary focus of the course is characterization of microorganisms based on their macroscopic and microscopic appearance, growth requirements, metabolic properties and proteins present in the cell wall. Additionally, students will try out methods routinely used in hospitals (microbial contamination monitoring, determination of microbial resistance to various antibiotics), procedures used in research laboratories (transformation of competent bacteria by plasmid) and in food industry (lactic fermentation resulting in products such yoghurt).

Last update: Ječmen Tomáš, RNDr., Ph.D. (18.02.2025)

Aim of the course

Cílem praktických cvičení je seznámit studenty se základními metodami používanými v mikrobiologii.

Last update: Ječmen Tomáš, RNDr., Ph.D. (06.05.2021)

Literature

Tomáš Ječmen, Věra Černá, Adriana Osičková, Roman Tuzhilkin - Practical Class in Biochemistry and Biology of Microorganisms (2024)

Last update: Ječmen Tomáš, RNDr., Ph.D. (18.02.2025)

Requirements to the exam

The conditions for granting of course credit:

1. Active and informed participation of the student.

1.1. Keeping a laboratory diary.

1.2. Understanding of the biochemical and microbiological concepts to the extent necessary to complete the individual tasks.

1.3. Compliance with the principles of occupational health and safety.

2. Elaboration of a protocol from one of the practical exercises within 14 days of the end of the practical part of the course. A protocol returned for revision must be corrected within 7 days.

Last update: Ječmen Tomáš, RNDr., Ph.D. (18.02.2025)

Syllabus

• Basic microbiological techniques (aseptic work, media preparation, inoculation)
• Staining and bright field microscopy of microorganisms
• Smear technique and counting of microorganisms
• Antibiotic susceptibility testing
• Growth curve of microorganisms
• Isolation and identification of bacteria in a mixture
• Metabolic identification tests
• Transformation of bacteria
• Production of fermented dairy products

Last update: Ječmen Tomáš, RNDr., Ph.D. (18.02.2025)

Entry requirements

• Attendance at the introductory meeting: 31 March 2025, 15:00, laboratory 201/203 (summer semester 2024/5).
• Passing the entrance test at the introductory meeting. The test covers occupational health and safety principles and biochemical/microbiological aspects relevant to the practical tasks described in the study material (see Files section). A brief description of key steps of one practical exercise will also be required in the test.
• Previous or parallel study of the subject Biochemistry and biology of microorganisms (MC250P08). [not required for Erasmus and PhD students]

Last update: Ječmen Tomáš, RNDr., Ph.D. (18.02.2025)