SubjectsSubjects(version: 953)
Course, academic year 2023/2024
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Molecular Imaging in Biomedicine - MC240P54
Title: Molekulární a buněčné zobrazování
Czech title: Molekulární a buněčné zobrazování
Guaranteed by: Department of Inorganic Chemistry (31-240)
Faculty: Faculty of Science
Actual: from 2021
Semester: summer
E-Credits: 3
Examination process: summer s.:
Hours per week, examination: summer s.:2/0, Ex [HT]
Capacity: unlimited
Min. number of students: 10
4EU+: no
Virtual mobility / capacity: no
State of the course: taught
Language: Czech, English
Note: enabled for web enrollment
Guarantor: Mgr. Vít Herynek, Ph.D.
Teacher(s): Mgr. Vít Herynek, Ph.D.
Annotation -
Molecular and cellular imaging (MI) in biomedical disciplines

Molecular and cellular imaging in biomedicine focuses on physical principles of imaging methods, chemical structure and synthesis of contrast agents and probes, and biological and clinical applications.
It covers common methods for in vivo a in vitro imaging, namely magnetic resonance imaging, MR spectroscopy, computer tomography, radionuclide methods, optical imaging, ultrasound and photoacoustic imaging. The methods provide besides a morphological information also pieces of knowledge about function and biochemical composition of the tissue, even on cellular and molecular level.
Lectures target both physical principles and certain applications.

Recommended for:
This course is intended for students of natural sciences, PhD students in biomedicine, neuroscience and other related fields.

Last update: Herynek Vít, Mgr., Ph.D. (28.01.2020)
Literature -
  • Vasilis Ntziachristos, Anne Leroy-Willig, Bertrand Tavitian (eds): Textbook of in vivo Imaging in Vertebrates, John Wiley & Sons 2007
  • Ján Weis, Peter Bořuta: Úvod do magnetickej rezonancie, Bratislava 1998
  • A. E. Merbach and É. Tóth eds.: The Chemistry of Contrast Agents in Medical Magnetic Resonance Imaging, John Wiley & Sons 2001.
Last update: Herynek Vít, Mgr., Ph.D. (12.01.2022)
Requirements to the exam -

The exam is combined - written and oral. Required knowledges are given by sylabus.

Last update: Kubíček Vojtěch, doc. RNDr., Ph.D. (26.10.2019)
Syllabus -

Molecular and cellular imaging in biomedicine (MI)

1. Historical overview of molecular imaging methods, their evolution, comparison (CT, MR, PET, SPECT, ultrasonography, optical imaging).

2. Methods of computer tomography (CT). Physical principles, construction. Native imaging, contrast agents.

3. Radionuclide methods. Physical principles, construction, workplace equipment. Gamma camera, SPECT, PET.

4. Fluorescence microscopy - imaging methods and biomedical applications.

5. Magnetic resonance (MR). Basic physical principles, resonance condition, chemical shift. Introduction to MR imaging, construction of MR spectrometers and tomographs.

6. MR imaging - T1 a T2 relaxation, contrast in MR image, relaxometry, contrast agents for MR – principles, structure.

7. In vivo MR spectroscopy – single voxel MR spectroscopy, spectroscopy imaging, processing of in vivo MR spectra. Nuclei used in in vivo MR spectroscopy, detectable metabolites and their biochemical pathways.

8. An overview of contrast agents for various imaging techniques. Direct measurement of molecules and their structure in vivo.

9. Magnetic particle imaging – physical principles, contrast agents.

10. Ultrasound and photoacoustic imaging – principles, applications in preclinical imaging.

11. Materials for cell and tissue transplantation.

12. Cell labelling for cell imaging, cell tracking and monitoring of the transplanted cell fate in vivo.

13. Combination of imaging methods, data co-registration.

14. Visit to the Center for Advanced Preclinical Imaging.

Last update: Kubíček Vojtěch, doc. RNDr., Ph.D. (28.12.2019)
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